| | 3 | Author
| Karin Groten, Wolfgang Barz | Requires cookie* | | | Title
| Elicitor-Induced Defence Reactions in Cell Suspension Cultures of Soybean Cultivars  | | | | Abstract
| Suspension cultured soybean (Glycine max [L.] Merr.) cells of four cultivars (Wilis, Lumut, Kalmit, Doko RC) were compared for their response to different fungal and bacterial elici tors. Cells were treated either with crude cell wall extracts of the fungal pathogens Phytophth ora sojae (Pmg-elicitor) and Rhizoctonia solani (Riso-elicitor) or with two isolates of the bacterial pathogen Pseudomonas syringae pv. glycinea (Psg01/02) and a broad spectrum of antimicrobial defence reactions was measured. Cells of all four cultivars showed the same elicitor-induced rapid (H20 2 accumulation, alkalinization of the culture medium, peroxida-tive cross-linking of cell wall proteins) and slow (activation of phenylpropanoid metabolism, accumulation of phenolic compounds, induction of PR-proteins) defence responses. How ever, the reactivity of the cultivars was not identical in terms of time courses and intensities. Furthermore, the ability of the various elicitors to induce defence responses varied markedly. These differences indicate that (1) cells of the same species but of different cultivars are equipped with the same array of perception systems to recognise various stimuli but (2) the sensitivity of these perception systems or later steps in the signal transduction seem to be stimulated to a different extent in the analysed cultivars. | | | |
Reference
| Z. Naturforsch. 55c, 718—730 (2000); received April 25/June 15 2000 | | | |
Published
| 2000 | | | |
Keywords
| Oxidative Burst, PR-Proteins, Phenylpropanoid Metabolism | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/55/ZNC-2000-55c-0718.pdf | | | | Identifier
| ZNC-2000-55c-0718 | | | | Volume
| 55 | |
| 4 | Author
| StefanL. Atza, RalfG B Erger | Requires cookie* | | | Title
| l-0-fra#is-Cinnamoyl-/?-D-glucopyranose: Alcohol Cinnamoyltransferase Activity in Fruits of Cape Gooseberry (Physalis peruviana L.) | | | | Abstract
| Methyl and ethyl cinnamate are aroma volatiles frequently occurring in fruits. Evidence was obtained that the enzymatic transfer of cinnamic acid to endogenous alcohols present in fruits (methanol, ethanol, 1-propanol) depended on energy-rich 1-O-glycosyl esters of cin namic acid which served as acyl donor molecules. A putative 1-O-fra/w-cinnamoyl-^-D-gluco-pyranose; alcohol cinnamoyltransferase from cape gooseberry (Physalis peruviana L.) was active towards l-0-rra«s-cinnamoyl-/3-D-glucopyranose and l-0-rrans-cinnamoyl-/3-D-gentio-biose. The enzyme was purified 290-fold by a protocol including ammonium sulphate precipi tation, solubilization by Triton X-100, gel permeation and affinity chromatography on conca-navalin A. The acidic glycoprotein {p i = 4.8) most probably is membrane bound. The distribution of alcohol cinnamoyltransferase activity in gel chromatography fractions suggests a native Mr of 75,000. For l-O-mms-cinnamoyl-ß-D-glucopyranose, an apparent K m of 69 ^im was determined. At pH > 6.0, non-enzymatic transesterification superposes the enzymatic transformation. | | | |
Reference
| Z. Naturforsch. 52c, 747—755 (1997); received June 12/July 28 1997 | | | |
Published
| 1997 | | | |
Keywords
| Phenylpropanoid Metabolism, Glycoconjugate, Flavour Precursor, Aroma Ester, Methyl Cinnamate | | | |
Similar Items
| Find | | | DEBUG INFO
| | | | | TEI-XML for
| default:Reihe_C/52/ZNC-1997-52c-0747.pdf | | | | Identifier
| ZNC-1997-52c-0747 | | | | Volume
| 52 | |
|
