| | 1 | Author
| W. N. Oé, H. U. Seitz | Requires cookie* | | | Title
| Studies on the Regulatory Role of fra/is-Cinnamic Acid on the Activity of the Phenylalanine Ammonia-Lyase (PAL) in Suspension Cultures of Daucus carota L  | | | | Abstract
| In vivo and in vitro experiments were performed in order to study the regulatory role of trans-cinnamic acid and its hydroxylated derivatives (p-coumaric acid, caffeic acid) on the deamina tion of phenylalanine catalyzed by PAL (EC 4.3.1.5). 7>arts-cinnamic acid inhibits growth and reduces the content of soluble proteins of anthocyanin-containing carrot cells grown in suspen sion. There is strong evidence from the polysomal patterns and from the effect of ?ra«5-cinnamic acid on protein synthesis in vitro that protein synthesis is inhibited. The kinetic data of PAL clearly demonstrate that /ra«s-cinnamic acid inhibits the enzyme by a noncompetitive mecha nism. On the contrary, L-a-aminooxy-/?-phenylpropionic acid (l-AOPP), a competitive inhibitor of PAL, does not affect protein metabolism. | | | |
Reference
| Z. Naturforsch. 38c, 408 (1983); received January 24 1983 | | | |
Published
| 1983 | | | |
Keywords
| Phenylalanine Ammonia-Lyase, Protein Metabolism, Regulation, /ra/js-Cinnamic Acid, Daucus carota, Suspension Culture | | | |
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| default:Reihe_C/38/ZNC-1983-38c-0408.pdf | | | | Identifier
| ZNC-1983-38c-0408 | | | | Volume
| 38 | |
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