| | 2 | Author
| Tjakko Stijve, Ruth Seeger | Requires cookie* | | | Title
| Determination of a-, ß and y-Amanitin by High Performance Thin-Layer Chromatography in Amanita phalloides (Vaill. ex Fr.) Seer, from Various Origin  | | | | Abstract
| A fast, sensitive high performance thin-layer chrom atographic method for the determ ination of a-, ß-, and y-amanitin in crude, m ethanolic extracts o f Amanita phalloides is described. The limit o f detection is 50 ng of each amanitin. W ith this method am anitin was determ ined in 24 pooled samples of Amanita phalloides, collect ed between 1970 and 1977 in G erm any and Switzerland. The total am anitin content varied be tween 2010 and 7300 mg/kg dry weight and the average value was 4430 m g/kg of which 43% was a-am anitin, 49% /?-amanitin and 8% y-amanitin. The origin of the fungi hardly influenced their am anitin content: in samples collected during the same year at different sites it fluctuated within a factor o f 1.7. The am anitin content of samples from the same site, but collected in different years, maximally varied within a factor o f 3.7. The partial decom position of am anitins during prolonged storage of the lyophilized samples undoubtedly contributed to this variation. Phalloidin, which was determined by conventional thin-layer-chrom atography, could not be de tected in a sample from 1970, whereas its concentration in m aterial collected during 1977 am ount ed to 2400 mg/kg dry weight. The toxicity of the samples (LD 50 o f lyophilized defatted methanolic extracts intravenously for mice) varied within a factor of 2.5. | | | |
Reference
| Z. Naturforsch. 34c, 1133—1138 (1979); received July 9 1979 | | | |
Published
| 1979 | | | |
Keywords
| Amanita phalloides, Amanitins, Phalloidin, M ushroom Poisoning, Chrom atography | | | |
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| default:Reihe_C/34/ZNC-1979-34c-1133.pdf | | | | Identifier
| ZNC-1979-34c-1133 | | | | Volume
| 34 | |
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