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'Petunia hybrida' in keywords
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1986 (1)
1985 (2)
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1983 (1)
1Author    J.M F G Aerts, A. W. SchramRequires cookie*
 Title    Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals I. A Comparison of Isolation Procedures  
 Abstract    Vacuoles were isolated from the upper epiderm is o f petals o f Petunia hybrida line R27 using three different procedures. U tilizing the vacuolar localization o f anthocyanin, vacuolar prep­ arations obtained through polybase treatment, osm otic shock and m echanical forces were com ­ pared on purity, yield and stability. The com parison indicated that application o f the polybase procedure results in the best vacuolar preparations. Vacuoles could be obtained which m ain­ tained their acidic pH and retained their anthocyanin content. 
  Reference    Z. Naturforsch. 40c, 189—1 (1985); received June 26/O ctober 26 1984 
  Published    1985 
  Keywords    Petunia hybrida, Vacuoles, Protoplasts, Anthocyanins 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0189.pdf 
 Identifier    ZNC-1985-40c-0189 
 Volume    40 
2Author    J.M F G Aerts, A. W. SchramRequires cookie*
 Title    Isolation of Vacuoles from the Upper Epidermis of Petunia Hybrida Petals ü . Vacuolar Localization of Some Hydrolases  
 Abstract    In vacuoles isolated from petal upper epiderm is o f Petunia hybrida through polybase-induced lysis o f protoplasts the vacuolar localization o f x-m annosidase and x-galactosidase was dem on­ strated. N o association was observed between the tonoplast and these hydrolases. Protoplasts from different mutant lines blocked in anthocyanin biosynthesis were treated with DEAE-dextran to release vacuoles. The differences in quality o f the obtained vacuolar preparations indicated the relation between mutant line used and successful application o f the polybase-procedure. 
  Reference    Z. Naturforsch. 40c, 196 (1985); received June 26/O ctober 26 1984 
  Published    1985 
  Keywords    Petunia hybrida, Vacuoles, Anthocyanins, Flavonoids, H ydrolases 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0196.pdf 
 Identifier    ZNC-1985-40c-0196 
 Volume    40 
3Author    A. W. Schram, L.M V Jonsson, P. De VlamingRequires cookie*
 Title    Identification of Anthocyanins and Intermediates of Anthocyanin Biosynthesis from Petunia hybrida Using High Performance Liquid Chromatography  
 Abstract    A procedure to identify dihydroflavonol (glucosides), cinnam ic acids and anthocyanins in flowers of Petunia hybrida is presented. Nearly all known interm ediates and anthocyanins ocur-ring in Petunia are separated on one column using different elution systems. Hydrolysis of extracts before HPLC and thin layer chrom atography is in some cases necessary to m ake positive identification of flavonoids. Anthocyanins in flowers o f Petunia hybrida can occur, depending on genotype, as acylated anthocyanins. Two types o f acylated anthocyanins have been identified differing in polarity and thus in retention times on HPLC and TLC. The difference in acylation is genetically deter­ mined and gives rise to m inor colour differences. 
  Reference    Z. Naturforsch. 38c, 342—345 (1983); received February 21 1983 
  Published    1983 
  Keywords    High Performance Liquid Chrom atography, Anthocyanins, Flavonoids, Cinnam ic Acids, Petunia hybrida 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0342.pdf 
 Identifier    ZNC-1983-38c-0342 
 Volume    38 
4Author    L.M V Jonsson, M.E G Aarsman, J. Bastiaannet, W.E D Onker-K, A.G M Oopm An, A. W. Geräts, SchramRequires cookie*
 Title    Common Identity of UDP-Glucose: Anthocyanidin 3-O-Glucosyltransferase and UDP-Glucose: Flavonol 3-O-Glucosyltransferase in Flowers of Petunia hybrida  
 Abstract    In an attempt to distinguish between the U D P -glucose: flavonol 3-O -glucosyltransferase (3G T) and the UD P-glucose:anthocyanidin 3-O-glucosyltransferase in flower buds o f Petunia hybrida, several properties o f these activities were determ ined. The 3-glucosylation o f anthocyanidin had a pH-activity optimum o f 7.2, that o f flavonol pH 9.2 to 9.5. A nthocyanidin 3 G T activity was lowered in the presence o f EDTA or /?-mercaptoethanol, but this was due to an effect on the anthocyanidin substrate. The two 3-glucosylating activities were to a sim ilar extent inhibited by an increasing ionic strength in the enzyme assay and showed an identical iso-electric point (5.2) as determined by chromatofocusing. M olecular weights were identical: 26000, 52000 or 78000 daltons as determined by gel-filtration. Antiserum raised against partially purified 3 G T gave identical immunoprecipitation curves with flavonol 3 G T and anthocyanidin 3G T . Special attention was given to the 3-O-glucosyltransferase in mutants with low levels o f 3 G T activity. These mutants are unable to form significant am ounts o f anthocyanins but contain wild-type amounts o f flavonols. The enzyme o f such mutants had the sam e iso-electric point and identical titration-curves with antiserum as the enzym e from w ildtype plants. 3 G T from w ildtype or mutant plants glucosylated flavonols at higher rates than anthocyanidins. 
  Reference    Z. Naturforsch. 39c, 559 (1984); received February 21 1984 
  Published    1984 
  Keywords    Petunia hybrida, Anthocyanins Flavonoid Biosynthesis, Flavonols, 3-O -G lucosyltransferase 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0559.pdf 
 Identifier    ZNC-1984-39c-0559 
 Volume    39 
5Author    G. Forkm, P. De Vlaming, R. Spribille, H. Wiering, A. W. SchramRequires cookie*
 Title    Genetic and Biochemical Studies on the Conversion of Dihydroflavonols to Flavonols in Flowers of Petunia hybrida  
 Abstract    Soluble enzyme preparations from flower buds of Petunia hybrida catalyzed the conversion of dihydroflavonols to flavonols. Dihydrokaempferol and dihydroquercetin were readily converted to the respective flavonols, whereas dihydromyricetin was a poor substrate. The reaction required 2-oxoglutarate, ascorbate and Fe2+ as cofactors and had a pH optimum at about 6.5. In the presence of the dominant allele FI, high enzyme activity for flavonol formation was found, whereas in enzyme preparations from flower buds of recessive genotypes (fl/fl) only low enzyme activity could be observed. A substantial correlation was found between enzyme activity for flavonol formation and the flavonol content of buds and flowers during development. 
  Reference    Z. Naturforsch. 41c, 179 (1986); received July 27/September 26 1985 
  Published    1986 
  Keywords    birthday Dioxygenase, Flavonoid Biosynthesis, Flavonols, Genetic Control, Petunia hybrida 
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 TEI-XML for    default:Reihe_C/41/ZNC-1986-41c-0179.pdf 
 Identifier    ZNC-1986-41c-0179 
 Volume    41