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'Petroselinum hortense' in keywords Facet   section ZfN Section C  [X]
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1988 (2)
1981 (1)
1Author    L. Britsch, W. Heller, H. GrisebachRequires cookie*
 Title    Conversion of Flavanone to Flavone, Dihydroflavonol and Flavonol with an Enzyme System from Cell Cultures of Parsley  
 Abstract    Soluble enzyme preparations from irradiated cell suspension cultures of parsley (Petroselinum hortense Hoffm.) catalyse the conversion o f flavanone to flavone, dihydroflavonol and flavonol. These reactions require 2-oxoglutarate, Fe2+ and ascorbate as cofactors. In the presence o f these cofactors conversion of dihydroflavonol to flavonol was also observed. With this system in vitro biosynthesis of radioactive flavone, dihydroflavonol and flavonol from [2-14C]malonyl-CoA and 4-coumaroyl-CoA in good yield and with high specific activity is possible. We postulate that synthesis of flavone and flavonol from flavanone proceeds via 2-hydroxy-and 2,3-dihydroxyflavanone, respectively, with subsequent dehydration. The microsomal fraction of the parsley cells contains an NADPH-dependent flavanone 3'-hydroxylase. 
  Reference    Z. Naturforsch. 36c, 742—750 (1981); received June 3 1981 
  Published    1981 
  Keywords    Flavonoids, Biosynthesis, Dioxygenases, Petroselinum hortense, Cell Cultures 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0742.pdf 
 Identifier    ZNC-1981-36c-0742 
 Volume    36 
2Author    Dieter Komoßa, Wolfgang BarzRequires cookie*
 Title    Glutaric Acid as a Catabolite of Nicotinic Acid in Parsley Cell Suspension Cultures  
 Abstract    A degradation product of nicotinic acid representing the pyridine carbon skeleton was isolated and purified from parsley cell suspension cultures after incubation with [6-14 C]nicotinic acid for 70 h. The catabolite was identified as glutaric acid by means of spectroscopic (GC-MS and 'H NMR) and chromatographic (TLC, HPLC) techniques. Glutaric acid when applied to parsley cell cultures was readily degraded to C0 2 but intermediate products could not be identified. 
  Reference    Z. Naturforsch. 43c, 843—849 (1988); received August 22 1988 
  Published    1988 
  Keywords    Nicotinic Acid, Degradation, Glutaric Acid, Petroselinum hortense, Cell Suspension Culture 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0843.pdf 
 Identifier    ZNC-1988-43c-0843 
 Volume    43 
3Author    Barbara Upmeier, JürgenE. Thomzik, Wolfgang BarzRequires cookie*
 Title    Enzymatic Studies on the Reversible Synthesis of Nicotinic Acid-N-glucoside in Heterotrophic Parsley Cell Suspension Cultures  
 Abstract    A soluble enzyme catalyzing the transfer of the glucose moiety from UDP-glucose to the nitrogen atom of nicotinic acid was detected in protein preparations from heterotrophic cell suspension cultures of parsley (Petroselinum hortense Hoffm.). Enzyme activity was enriched 22-fold by ammonium sulfate precipitation, gel filtration and ion exchange chromatography. The UDP-glucose : nicotinic acid-N-glucosyltransferase showed a pH-optimum at pH 7.8—8.2 and a temperature optimum at 30 °C. The apparent K M values were determined to be 170 ^M for nicotinic acid and 1.2 mM for the cosubstrate. The native enzyme had a molecular mass of about 46 kDa. The glucosyltransferase reaction was shown to be reversible. The transfer of the glucose molecule from nicotinic acid-N-glucoside to uridinediphosphate yielding uridinediphosphoglucose and nicotinic acid could be demonstrated indicating that nicotinic acid-N-glucoside has a high group-transfer potential. 
  Reference    Z. Naturforsch. 43c, 835—842 (1988); received August 1/September 30 1988 
  Published    1988 
  Keywords    Petroselinum hortense, Umbelliferae, Plant Cell Cultures, Nicotinic Acid, Nicotinic Acid-N-glucoside 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0835.pdf 
 Identifier    ZNC-1988-43c-0835 
 Volume    43