| 2 | Author
| Barbara Upmeier, JürgenE. Thomzik, Wolfgang Barz | Requires cookie* | | Title
| Enzymatic Studies on the Reversible Synthesis of Nicotinic Acid-N-glucoside in Heterotrophic Parsley Cell Suspension Cultures  | | | Abstract
| A soluble enzyme catalyzing the transfer of the glucose moiety from UDP-glucose to the nitrogen atom of nicotinic acid was detected in protein preparations from heterotrophic cell suspension cultures of parsley (Petroselinum hortense Hoffm.). Enzyme activity was enriched 22-fold by ammonium sulfate precipitation, gel filtration and ion exchange chromatography. The UDP-glucose : nicotinic acid-N-glucosyltransferase showed a pH-optimum at pH 7.8—8.2 and a temperature optimum at 30 °C. The apparent K M values were determined to be 170 ^M for nicotinic acid and 1.2 mM for the cosubstrate. The native enzyme had a molecular mass of about 46 kDa. The glucosyltransferase reaction was shown to be reversible. The transfer of the glucose molecule from nicotinic acid-N-glucoside to uridinediphosphate yielding uridinediphosphoglucose and nicotinic acid could be demonstrated indicating that nicotinic acid-N-glucoside has a high group-transfer potential. | | |
Reference
| Z. Naturforsch. 43c, 835—842 (1988); received August 1/September 30 1988 | | |
Published
| 1988 | | |
Keywords
| Petroselinum hortense, Umbelliferae, Plant Cell Cultures, Nicotinic Acid, Nicotinic Acid-N-glucoside | | |
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| default:Reihe_C/43/ZNC-1988-43c-0835.pdf | | | Identifier
| ZNC-1988-43c-0835 | | | Volume
| 43 | |
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