ZfN: Search Results

1	Author	Dieter Komoßa, Wolfgang Barz	Requires cookie*
	Title	Glutaric Acid as a Catabolite of Nicotinic Acid in Parsley Cell Suspension Cultures
	Abstract	A degradation product of nicotinic acid representing the pyridine carbon skeleton was isolated and purified from parsley cell suspension cultures after incubation with [6-14 C]nicotinic acid for 70 h. The catabolite was identified as glutaric acid by means of spectroscopic (GC-MS and 'H NMR) and chromatographic (TLC, HPLC) techniques. Glutaric acid when applied to parsley cell cultures was readily degraded to C0 2 but intermediate products could not be identified.
	Reference	Z. Naturforsch. 43c, 843—849 (1988); received August 22 1988
	Published	1988
	Keywords	Nicotinic Acid, Degradation, Glutaric Acid, Petroselinum hortense, Cell Suspension Culture
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	TEI-XML for	default:Reihe_C/43/ZNC-1988-43c-0843.pdf
	Identifier	ZNC-1988-43c-0843
	Volume	43

2	Author	Barbara Upmeier, JürgenE. Thomzik, Wolfgang Barz	Requires cookie*
	Title	Enzymatic Studies on the Reversible Synthesis of Nicotinic Acid-N-glucoside in Heterotrophic Parsley Cell Suspension Cultures
	Abstract	A soluble enzyme catalyzing the transfer of the glucose moiety from UDP-glucose to the nitrogen atom of nicotinic acid was detected in protein preparations from heterotrophic cell suspension cultures of parsley (Petroselinum hortense Hoffm.). Enzyme activity was enriched 22-fold by ammonium sulfate precipitation, gel filtration and ion exchange chromatography. The UDP-glucose : nicotinic acid-N-glucosyltransferase showed a pH-optimum at pH 7.8—8.2 and a temperature optimum at 30 °C. The apparent K M values were determined to be 170 ^M for nicotinic acid and 1.2 mM for the cosubstrate. The native enzyme had a molecular mass of about 46 kDa. The glucosyltransferase reaction was shown to be reversible. The transfer of the glucose molecule from nicotinic acid-N-glucoside to uridinediphosphate yielding uridinediphosphoglucose and nicotinic acid could be demonstrated indicating that nicotinic acid-N-glucoside has a high group-transfer potential.
	Reference	Z. Naturforsch. 43c, 835—842 (1988); received August 1/September 30 1988
	Published	1988
	Keywords	Petroselinum hortense, Umbelliferae, Plant Cell Cultures, Nicotinic Acid, Nicotinic Acid-N-glucoside
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	TEI-XML for	default:Reihe_C/43/ZNC-1988-43c-0835.pdf
	Identifier	ZNC-1988-43c-0835
	Volume	43