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'Petroselinum crispum' in keywords Facet   section ZfN Section C  [X]
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1990 (1)
1979 (1)
1Author    Ursula Seitz, Ulrich SeitzRequires cookie*
 Title    The Molecular Weight of rRNA Precursor Molecules and Their Processing in Higher Plant Cells  
 Abstract    Actively dividing callus cells of higher plants (Petroselinum crispum, Daucus carota, Acer pseudoplatanus) were used to detect the primary gene product of rDNA in vivo. Parsley and carrot cells were labelled with [32P] orthophosphate. Under non-denaturing conditions, in both cases only one high molecular weight rRNA precursor was present on polyacrylamide gels. Its molecular weight did not exceed 2.5X10® dalton. Under denaturing conditions, 2.0 — 2.1X10® dalton were determined on formamide gels. This rRNA precursor was already present after a labelling period of 5 —10 min. In parsley cells labelled mature rRNA (25S and 18S) arrived in the cytoplasm 45 min after onset of incubation. In Acer pseudoplatanus incubated with [3H] uridine two rapidly labelled components did emerge from polyacrylamide gels without formamide; their molecular weights were 2.3 and 3.2 — 3.4X10® dalton. After electrophoresis in formamide, the larger component disappeared, thus indicating that it would be an intermolecular aggregate of different for the existence of rRNA precursors exceeding the 
  Reference    Z. Naturforsch. 34c, 253 (1979); received January 23 1979 
  Published    1979 
  Keywords    Petroselinum crispum, Daucus carota, Acer pseudoplatanus, Callus Cells, rRNA Precursor 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0253.pdf 
 Identifier    ZNC-1979-34c-0253 
 Volume    34 
2Author    Joachim Bollmann, Klaus HahlbrockRequires cookie*
 Title    Timing of Changes in Protein Synthesis Pattern in Elicitor-Treated Cell Suspension Cultures of Parsley ( Petroselinum crispum)  
 Abstract    The timing o f changes in the protein synthesis pattern o f elicitor-treated, [35S]methionine-labelled parsley cells (Petroselinum crispum) was analyzed by two-dimensional gel electropho­ resis. Five groups were distinguished from a large number o f elicitor-responsive as well as un­ responsive proteins. Two groups were synthesized de novo either early or late after elicitor ap­ plication; two other groups were strongly reduced in their rates o f synthesis either early or late after elicitor application; and one group was not appreciably affected at all. The elicitor-in-duced changes altered the total protein com position considerably. A few selected, induced pro­ teins were functionally identified. These included two early induced enzymes, phenylalanine amm onia-lyase (PAL) and 4-coum arate:C oA ligase (4C L), and a late induced enzyme, a ber-gaptol O-methyltransferase (BM T) which is specifically involved in the biosynthesis o f furano-coumarin phytoalexins. The biological significance o f the observed differential timing o f changes in protein synthesis rates is discussed. 
  Reference    Z. Naturforsch. 45c, 1011 (1990); received May 30 1990 
  Published    1990 
  Keywords    Cell Culture, Elicitor, Petroselinum crispum, Protein Synthesis, Two-Dim ensional Gel Electrophoresis 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-1011.pdf 
 Identifier    ZNC-1990-45c-1011 
 Volume    45