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'Permeabilized Cells' in keywords Facet   section ZfN Section C  [X]
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1989 (1)
1Author    Jochen Berlin, BerndM. Artin, Jerzy Nowak, LudgerW. Itte+, Victor Wray, D. Ieter Strack+, G. Esellschaft Für, Biotechnologische Forschung, BhM. Ascheroder, W. EgRequires cookie*
 Title    Effects of Permeabilization on the Biotransformation of Phenylalanine by Immobilized Tobacco Cell Cultures  
 Abstract    Im m obilized tobacco cells excreted only a small proportion of their main secondary m etabolite caffeoylputrescine into the culture m edium . Experim ents designed to release more o f the com ­ pound by perm eabilization caused the loss o f caffeoylputrescine, probably by oxidative reactions. M oreover, rather mild treatments with pejm eabilizing agents (e.g. «-propanol) resulted in severe growth inhibition. The ability of perm eabilized cells to form caffeoylputrescine and other hy-droxycinnam oyl conjugates from phenylalanine decreased considerably even when such cells were still able to m etabolize phenylalanine into various ethyl acetate extractable com pounds (e.g. hydroxycinnamic acids and acetophenones). The formation of new biotransformation products suggests that perm eabilized cells could be used as a tool for testing the enzymatic capabilities o f a cell culture. 
  Reference    Z. Naturforsch. 44c, 249 (1989); received D ecem ber 5 1988 
  Published    1989 
  Keywords    Nicotiana tabacum, Cell Suspension Cultures, Imm obilized Cells, Permeabilized Cells, Biotrans­ form ation, Phenylalanine 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0249.pdf 
 Identifier    ZNC-1989-44c-0249 
 Volume    44