| 3 | Author
| Hubert Kalbacher, Claus Bürvenich, Stefan Fuchs, Hans Horn, Wolfgang Klingler, Emanuel Pietrzik, Karl Zech, Wolfgang Voelter | Requires cookie* | | Title
| Totalsynthese von Somatostatin, I Total Synthesis of Somatostatin, I  | | | Abstract
| Experimental details of the syntheses of five peptide fragments, used for the total synthesis of somatostatin, are described. N-Hydroxysuccinimide esters and DCC are used for forming the peptide bonds. The amino acid side chains are protected by trityl, tert-butyloxycarbonyl and benzyl groups. | | |
Reference
| (Z. Naturforsch. 31b, 1702—1707 [1976]; eingegangen am 28. September 1976) | | |
Published
| 1976 | | |
Keywords
| Peptide Synthesis, Peptide Hormones, Hypothalamus-releasing Hormones, Somatostatin | | |
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| default:Reihe_B/31/ZNB-1976-31b-1702.pdf | | | Identifier
| ZNB-1976-31b-1702 | | | Volume
| 31 | |
4 | Author
| Anders Ljungqvist, Karl Folkers | Requires cookie* | | Title
| Study of Hydrogenolytic Cleavage of Peptide-Resin Benzyl Ester Bonds for Synthesis of Protected Fragments of the Human Leukocyte Interferon  | | | Abstract
| Three Boc-protected fragments of the human leukocyte interferon, LeIFA, have been synthesized: I, BOC LeIFA (99-105) (BOC-Val-Ile-Gln-Gly-Val-Gly-Val); II, BOC LeIFA (116-119) (BOC-Ile-Leu-Ala-Val); III, BOC LeIFA (123-126) (BOC-Phe-Gln-Arg(Tos)-Ile). The presence of the Boc-group was desired so that these peptides could serve as intermediates in fragment condensation toward larger segments of interferon. To achieve these intermediates, a study was desirable on three methods of cleavage of the benzyl ester-polymer bonds. It was found that hydrogenolysis with hydrogen and palla-dium generated in situ was distinctly superior to transfer hydrogenation with ammonium formate or cyclohexene. An effective swelling of the peptide-resin to allow penetration of palladium acetate into the matrix and mobility of the peptide chains on the resin to expose the benzyl ester bond to hydrogenolysis appear to be essential conditions for the best cleavage. | | |
Reference
| Z. Naturforsch. 38b, 1022—1024 (1983); received May 2 1983 | | |
Published
| 1983 | | |
Keywords
| Interferon, Peptide Synthesis, Hydrogenation, Palladium Black, Conformation | | |
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| default:Reihe_B/38/ZNB-1983-38b-1022.pdf | | | Identifier
| ZNB-1983-38b-1022 | | | Volume
| 38 | |
8 | Author
| Anders Ljungqvist, Karl Folkers+ | Requires cookie* | | Title
| Synthesis of a Combined Fragment of Interferon, AeHuIFN a A(30-43)-(123-137)-NH2 Interferon May not be a Sychnologieal Peptide * AeHuIFN a A( 30-43)-( 123-137)-NH2, Ac-Leu-Lys-Asp-Arg-His-Asp-Phe-Gly-Phe- Pro-Gln-Glu-Glu-Phe-Phe-Gln-Arg-Ile-Thr-Leu-Tyr-Leu-Lys-Glu-Lys-Lys-Tyr-Ser- Pro-NH2 was  | | | Abstract
| synthesized toward encompassing the "active site" of the interferons. The design of this 29-amino acid peptide was based on considerations of homology between the different interferons and on combining two regions of interferons, and on known and predicted structural features. The peptide was characterized by amino acid analysis, thin layer chromatography in several systems, and by HPLC. As tested, the peptide neither showed antiviral activity nor blocked antiviral activity of interferon, indicating that the active site was not encompassed or that interferon may not be a sychnologieal peptide. | | |
Reference
| Z. Naturforsch. 38b, 1249—1252 (1983); received June 24 1983 | | |
Published
| 1983 | | |
Keywords
| Interferon, Peptide Synthesis, Antiviral Activity, Synthetic Fragment, Active Site | | |
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| default:Reihe_B/38/ZNB-1983-38b-1249.pdf | | | Identifier
| ZNB-1983-38b-1249 | | | Volume
| 38 | |
9 | Author
| B. Ernd, H. Enkel, L.Ianshan Zhang, C. Arsten, G. Oldam, M. Er, E.Rnst Bayer | Requires cookie* | | Title
| Combined Solid Phase and Solution Synthesis of the Fully Protected Segment 74-99 of HIV 1-Protease with the Application of a New Trityl-Linker  | | | Abstract
| A new trityl-linker for the strategy of combined solid phase and solution synthesis has been used for the synthesis of the fully protected segment 74-99 of HIV 1-protease. The fully protected fragments 74-81, 82-99 and 90-99 have been synthesized on the polystyrene-polyethylenglykol resin TentaGel, split off with dilute acetic acid and assembled in solution without loss of side-chain protecting groups. After protection of the C-terminus of the frag ment 90-99, the second fragment was coupled with TBTU/NMM. The couplings were moni tored by IS-MS and HPLC. Racemization was checked by chiral gas-chromatography on a Chirasil-Val capillary. | | |
Reference
| Z. Naturforsch. 51b, 1339—1346 (1996); received April 10 1996 | | |
Published
| 1996 | | |
Keywords
| HIV 1-Protease, Trityl-Linker, Fragment Condensation, Peptide Synthesis, Ionspray-MS, Chiral Gas-chromatography | | |
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| default:Reihe_B/51/ZNB-1996-51b-1339.pdf | | | Identifier
| ZNB-1996-51b-1339 | | | Volume
| 51 | |
10 | Author
| Yuejin Lia, Lianshan Zhangb, Ernst Bayerb, Dieter Oelkrugc, Ulrich Wesera | Requires cookie* | | Title
| Comparative Luminescence of Rat Liver Cu-thionein and Its Chemically Synthesized a-Domain  | | | Abstract
| A peptide corresponding to the a-dom ain o f rat liver m etallothionein-2 was chemically syn thesized employing the solid phase peptide synthesis technique. Its luminescence properties that depend on the coordinated Cu(I) have been studied using luminescence spectrometric titration in the presence o f Cu(I). Unlike the intact metallothionein which has been converted into the Cu species, the emission and excitation spectra o f the Cu-a-fragm ent showed a red shift by 20 nm and 65 nm, respectively, suggesting a more compact and stable luminophore in the a-dom ain. Saturation o f Cu(I) coordination was reached in the presence o f 6.5 mol eq Cu(I) when the a-fragment was used and 12 mol eq Cu(I) were specifically bound by the intact metallothionein. The emission bands were hom ogeneous and no decline o f the cluster struc ture was observed when excessive Cu(I) was added after saturation. A rearrangement o f the Cu-cluster in metallothionein during its formation seems to be plausible. | | |
Reference
| Z. Naturforsch. 45c, 1193—1196 (1990); received June 6/A ugust 21 1990 | | |
Published
| 1990 | | |
Keywords
| a-Fragment o f M etallothionein, Luminescence, Cu-Binding, Solid Phase, Peptide Synthesis | | |
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| default:Reihe_C/45/ZNC-1990-45c-1193.pdf | | | Identifier
| ZNC-1990-45c-1193 | | | Volume
| 45 | |
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