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1999 (1)
1Author    Narayanan RajendranRequires cookie*
 Title    Identification and Cloning of a Gene Locus Encoding Peptide Synthetase of Pseudomonas fluorescens by Two Sets of PCR Primers  
 Abstract    A chromosomal locus encoding biosynthetic genes for a putative peptide synthetase of Pseudomonas fluorescens was identified and cloned. To achieve this, two sets of degenerated oligonucleotide primers KAGGA:SGTTG and TG D :LG G were used in PCR. These primers were selected based on highly conserved units of known peptide synthetases involved in adenylation and thiolation regions of Bacillus subtilis. The discrete amplified bands from PCR ca. 300 bp for KAGGA:SGTTG and ca. 500 bp for TGDrLGG proved to be integral part of the genomic DNA of P. fluorescens were cloned and sequenced. Sequence alignments of both fragments confirmed the putative peptide synthetase genes in P. fluorescens. The present study describes the identification and cloning of peptide synthetase genes of P. flu o ­ rescens, which can be used to identify a genetic locus encoding peptide synthetase in other microbial species. 
  Reference    Z. Naturforsch. 54c, 105—109 (1999); received August 26/September 21 1998 
  Published    1999 
  Keywords    Oligonucleotide Primers, Pseudomonas fluorescens, Peptide Synthetase, Identification 
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 TEI-XML for    default:Reihe_C/54/ZNC-1999-54c-0105.pdf 
 Identifier    ZNC-1999-54c-0105 
 Volume    54