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1985 (1)
1Author    Friedrich, C. Hristian, B. Aum AnnRequires cookie*
 Title    A Patch Clamp Study of Tonoplast Electrical Properties in Vacuoles Isolated from Chenopodium rubrum Suspension Cells  
 Abstract    -W ilhelm B en tru p , B ernd H offm ann , M aria G o g arten -B o ek els, Jo h a n n P ete r G ogarten The patch clamp technique has been applied to isolated vacuoles from green suspension cells of Chenopodium rubrum to record electrical parameters of the tonoplast. In a symmetrical K* solution of 46 mM, the membrane displays a near zero voltage, whereas 2 mM ATP will hyper-polarize it to 15 or 20 mV (vacuole positive). The conductance amounts to about one S-m~2. Fluctuations of the clamp current are explained by an unknown channel species having opening times of 5 — 10 ms. Together with previous work on a tonoplast vesicle preparation and unpub­ lished data on vacuoles from our laboratory, the present results indicate an electrogenic mem­ brane ATPase pumping protons from the cytoplasm to the vacuole. 
  Reference    Z. Naturforsch. 40c, 886 (1985); received October 23 1985 
  Published    1985 
  Keywords    Patch Clamp, Membrane ATPase, Tonoplast, Vacuole, Chenopodium rubrum L 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0886.pdf 
 Identifier    ZNC-1985-40c-0886 
 Volume    40