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'Partial Hydrolysis' in keywords Facet   section ZfN Section C  [X]
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1980 (1)
1Author    D. Ieter, S. Track3, N. Aam, A. Kaviab, HansR. EznikaRequires cookie*
 Title    High Performance Liquid Chromatographic Identification of Anthocyanins  
 Abstract    A high performance liquid chromatographic procedure for a rapid identification o f antho­ cyanins is described. Besides their characteristic retention times, anthocyanins can easily be identified by an HPLC examination o f the kinetics of appearance and disappearance o f their partial hydrolysis products. This method is also applicable to 3-deoxyanthocyanins which is demonstrated with an extract from the fern Blechnum brasiliense. The described method allows optimal resolution of complex anthocyanin mixtures in the fol­ lowing sequence o f elution: 3-sophoroside-5-glucoside; 3-rutinoside-5-glucoside; 3,5-diglucoside; 3-sophoroside; 3-galactoside; 3-glucoside; 3-rutinoside; 5-glucoside; aglycone, and finally the forms acylated with /?-coumaric acid. Substitution in the B-ring o f the aglycone structure is the key factor for the range o f the retention time. Thus cyanidin 3,5-diglucoside elutes earlier (—38 sec) than pelargonidin 3-sophoroside-5-glucoside. 
  Reference    Z. Naturforsch. 35c, 533—538 (1980); received March 20 1980 
  Published    1980 
  Keywords    Anthocyanins, 3-Deoxyanthocyanins, Partial Hydrolysis, High Performance Liquid Chromato­ graphy 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0533.pdf 
 Identifier    ZNC-1980-35c-0533 
 Volume    35