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'Partial Hepatectomy' in keywords Facet   Publication Year 1979  [X]
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1Author    Paul Miller, W. Alfried, A. Linden, Claudio NicoliniRequires cookie*
 Title    Physico-Chemical Studies of Isolated Chromatin Compared with in situ Chromatin after Partial Hepatectomy in the Rat  
 Abstract    Chromatin was isolated from rat liver cells at 0, 3, 5, 11, 18 and 24 h following partial hepa­ tectomy. Consistent with findings in cultured cells stimulated to proliferate, there was an increase in chromatin molar ellipticity measured at 276 nm, and a decrease in thermal stability 3 to 8 h after surgery. These events occured prior to the onset of DNA synthesis. These early changes be­ tween non-proliferating (G0) and proliferating (Gj) cells, as well as later chromatin conformational changes observed at S and G2 phases, mimic changes in template activity. Results with sheared and unsheared chromatin (both with in vitro and in vivo systems) prove that structural and functional changes can be caused by even the slightest shearing during chromatin preparation, suggesting the loss of native chromatin organization. To eliminate this problem, ex­ periments were also conducted using chromatin in situ. A flow cytometer (FCM) was used to study unfixed liver cell suspensions stained with ethidium bromide (EB). Fluorescence was mea­ sured in the green spectral range after addition of increasing amounts of EB. Experimental evidence is provided that the same alteration in chromatin conformation can be best detected using low molar ratios of EB per unit DNA due to greater fluorescence emission in Gj respect to G0 cells. These correlated studies demonstrate that the same changes controlling chromatin organization in situ are detected also in the tertiary-quaternary structure of "isolated" chromatin. These changes in chromatin conformation are macromolecular events related to cell proliferation both at the G0 —Gt and Gt —S transitions. 
  Reference    Z. Naturforsch. 34c, 442 (1979); received December 15 1978/February 19 1979 
  Published    1979 
  Keywords    Isolated Chromatin, Partial Hepatectomy, Molar Ellipticity, Thermal Stability, Flow Cytometry 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0442.pdf 
 Identifier    ZNC-1979-34c-0442 
 Volume    34