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2001 (1)
2000 (1)
1Author    Karin Groten, Wolfgang BarzRequires cookie*
 Title    Elicitor-Induced Defence Reactions in Cell Suspension Cultures of Soybean Cultivars  
 Abstract    Suspension cultured soybean (Glycine max [L.] Merr.) cells of four cultivars (Wilis, Lumut, Kalmit, Doko RC) were compared for their response to different fungal and bacterial elici­ tors. Cells were treated either with crude cell wall extracts of the fungal pathogens Phytophth­ ora sojae (Pmg-elicitor) and Rhizoctonia solani (Riso-elicitor) or with two isolates of the bacterial pathogen Pseudomonas syringae pv. glycinea (Psg01/02) and a broad spectrum of antimicrobial defence reactions was measured. Cells of all four cultivars showed the same elicitor-induced rapid (H20 2 accumulation, alkalinization of the culture medium, peroxida-tive cross-linking of cell wall proteins) and slow (activation of phenylpropanoid metabolism, accumulation of phenolic compounds, induction of PR-proteins) defence responses. How­ ever, the reactivity of the cultivars was not identical in terms of time courses and intensities. Furthermore, the ability of the various elicitors to induce defence responses varied markedly. These differences indicate that (1) cells of the same species but of different cultivars are equipped with the same array of perception systems to recognise various stimuli but (2) the sensitivity of these perception systems or later steps in the signal transduction seem to be stimulated to a different extent in the analysed cultivars. 
  Reference    Z. Naturforsch. 55c, 718—730 (2000); received April 25/June 15 2000 
  Published    2000 
  Keywords    Oxidative Burst, PR-Proteins, Phenylpropanoid Metabolism 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0718.pdf 
 Identifier    ZNC-2000-55c-0718 
 Volume    55 
2Author    Thomas Hanselle3, Yuki Ichinosebb, Wolfgang Barz3Requires cookie*
 Title    Biochemical and Molecular Biological Studies on Infection (Ascochyta rabiei)- Induced Thaumatin-Like Proteins from Chickpea Plants (Cicer arietinum L.)  
 Abstract    Tel.: + 4 9 -2 5 1 -8 3 2 4 7 9 1 . Fax: + 4 9 -2 5 1 -8 3 2 8 3 7 1 . A pathogenesis-related protein induced by infection with Ascochyta rabiei was purified from intercellular washing fluid of chickpea (Cicer arietinum L.) leaves. Amino-terminal se­ quencing identified the protein, named PR-5a, as a thaumatin-like protein. The isoelectric point was determined with 6.5 and the molecular mass is 16 kDa. Therefore, chickpea PR-5a is the first dicot member of a TLP subgroup containing small TLPs with a molecular weight between 15 and 18 kDa. PR-5a shows no antifungal activity towards A. rabiei. Screen­ ing of a chickpea cDNA library led to the isolation of a cDNA clone (p5a-241) for this protein. A second cDNA clone (E L R 112) encoding a TLP was isolated using differential hybridisation of cDNA libraries obtained from elicited and water treated cell suspension cultures of chickpea. The deduced protein (PR-5b) has a molecular mass of 22 kDa. PR-5b is postulated to be located in the vacuole due to the presence of a respective N-terminal signal peptide and a carboxy-terminal extension. Southern blot analyses showed that E LR 112 and p5a-241 represent single copy genes. During fungal infection of chickpea plants expres­ sion of both genes proceeds much faster in an A. rabiei resistant cultivar than in a suscep­ tible one. 
  Reference    Z. Naturforsch. 56c, 1095—1107 (2001); received April 20/July 2 2001 
  Published    2001 
  Keywords    Cultivar Specific Expression, PR-Proteins, Thaumatin-Like Protein 
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 TEI-XML for    default:Reihe_C/56/ZNC-2001-56c-1095.pdf 
 Identifier    ZNC-2001-56c-1095 
 Volume    56