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'Oxidoreductases' in keywords Facet   Publication Year 1981  [X]
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1981[X]
1Author    Rainer Jaenicke, Hans-Dietrich Liidemann, Gerhard SchmidRequires cookie*
 Title    Pressure, Temperature and pH Dependence of the Absorption Spectrum of Reduced Nicotinamide Adenine Dinucleotide  
 Abstract    Enzymological studies at high hydrostatic pressure generally involve temperature, pH and pressure as variables, owing to the effect of adiabatic compression and the ionization volume o f the buffer system. In the case of N AD dependent oxidoreductases this implies that the extinction coefficient o f the coenzyme may be affected by p, T and pH, apart from the spectral change accompanying the redox reaction. Measurements o f the pressure dependence of the absorbance of N AD H show a slight red shift and a 1% decrease (3% increase) o f the absorbance at 339 nm (360 nm) at 2 kbar. The pH depen­ dence at the given wavelengths amounts to —(2.4 ± 0.1)% per pH unit (25 °C), while the intrinsic temperature effect (after correction for thermal expansion) is o f the order o f -0.2% per degree (2 0 -3 0 °C). Applying buffers with negligible ionization volume, 366 nm is the optimum wavelength for high pressure studies up to 2 kbar because here the pressure dependent spectral changes o f the N ADH absorption vanish. 
  Reference    Z. Naturforsch. 36c, 84—8 (1981); received October 9 1980 
  Published    1981 
  Keywords    Absorption, Dehydrogenases, High Pressure, NADH, Oxidoreductases, pH Dependence, Tem­ perature Dependence 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0084.pdf 
 Identifier    ZNC-1981-36c-0084 
 Volume    36