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2001 (1)
2000 (1)
1Author    Karin Groten, Wolfgang BarzRequires cookie*
 Title    Elicitor-Induced Defence Reactions in Cell Suspension Cultures of Soybean Cultivars  
 Abstract    Suspension cultured soybean (Glycine max [L.] Merr.) cells of four cultivars (Wilis, Lumut, Kalmit, Doko RC) were compared for their response to different fungal and bacterial elici­ tors. Cells were treated either with crude cell wall extracts of the fungal pathogens Phytophth­ ora sojae (Pmg-elicitor) and Rhizoctonia solani (Riso-elicitor) or with two isolates of the bacterial pathogen Pseudomonas syringae pv. glycinea (Psg01/02) and a broad spectrum of antimicrobial defence reactions was measured. Cells of all four cultivars showed the same elicitor-induced rapid (H20 2 accumulation, alkalinization of the culture medium, peroxida-tive cross-linking of cell wall proteins) and slow (activation of phenylpropanoid metabolism, accumulation of phenolic compounds, induction of PR-proteins) defence responses. How­ ever, the reactivity of the cultivars was not identical in terms of time courses and intensities. Furthermore, the ability of the various elicitors to induce defence responses varied markedly. These differences indicate that (1) cells of the same species but of different cultivars are equipped with the same array of perception systems to recognise various stimuli but (2) the sensitivity of these perception systems or later steps in the signal transduction seem to be stimulated to a different extent in the analysed cultivars. 
  Reference    Z. Naturforsch. 55c, 718—730 (2000); received April 25/June 15 2000 
  Published    2000 
  Keywords    Oxidative Burst, PR-Proteins, Phenylpropanoid Metabolism 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0718.pdf 
 Identifier    ZNC-2000-55c-0718 
 Volume    55 
2Author    OliverO. Tte, Andreas Pachten, Frauke Hein, Wolfgang BarzRequires cookie*
 Title    Early Elicitor-Induced Events in Chickpea Cells: Functional Links between Oxidative Burst, Sequential Occurrence of Extracellular Alkalinisation and Acidification, K+/H + Exchange and Defence-Related Gene Activation  
 Abstract    Elicitation of cultured chickpea (Cicer arietinum L.) cells stimulates a signal transduction pathway leading to several rapid responses: (1) oxidative burst, (2) extracellular alkalinisa­ tion, (3) extracellular acidification, (4) transient K+ efflux, and (5) activation of defence related genes all within 2 hours. Induced genes are encoding acidic and basic chitinases, a thaumatin-like protein and isoflavone reductase. All these elicitor-induced responses are in­ hibited by the Ser/Thr protein kinase inhibitor staurosporine and the anion channel blocker anthracene-9-carboxylic acid but stimulated by the Ser/Thr protein phosphatase 2A inhibitor cantharidin. The oxidative burst leads to a transient extracellular H2 0 2 accumulation which seems to be preceded by 0 2~ production, indicating dismutation of 0 2~ to H2 0 2. The oxida­ tive burst is accompanied by transient alkalinisation of the culture medium which is followed by long-lasting extracellular acidification. An 80 percent inhibition of the alkalinisation after complete inhibition of the H2 0 2 burst with diphenylene iodonium indicates that the elicitor induced increase of extracellular pH is mainly based on a proton consumption for 0 2~ dismutation. A simultaneous deactivation of the plasma membrane H+-ATPase during oxida­ tive burst and extracellular alkalinisation is also suggested. The elicitor-stimulated extracellu­ lar acidification is inhibited by the plasma membrane H+-ATPase inhibitor N, N'-dicyclohex-ylcarbodiimide assuming a reactivation of the H+-ATPase 25 min after elicitation. Extracellular acidification seems not to be necessary for elicitor-induced activation of defence related genes. Opposite modulation of K+ and proton fluxes after elicitation and/or treatment with the H +-ATPase effectors fusicoccin or N, N'-dicyclohexylcarbodiimide indicate that the elicitor induced transient K+ efflux is regulated by a K+/H+ exchange reaction. 
  Reference    Z. Naturforsch. 56c, 65—76 (2001); received August 29 2000 
  Published    2001 
  Keywords    Cicer arietinum, Defence Related Gene Activation, Extracellular Acidification, Oxidative Burst 
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 TEI-XML for    default:Reihe_C/56/ZNC-2001-56c-0065.pdf 
 Identifier    ZNC-2001-56c-0065 
 Volume    56