| | 1 | Author
| UweJ. Jürgens, Roland Benz | Requires cookie* | | | Title
| Pore-Forming Activity of Outer Membrane Extracts from the Unicellular Cyanobacterium Synechocystis sp. PCC 6714  | | | | Abstract
| Cell walls of the unicellular cyanobacterium Synechocystis sp. PCC 6714, isolated from cell homogenates, were found to be unusually resistant against extraction with various detergents, organic solvents, chaotropic agents, and proteases. The major outer membrane proteins (M r 67,000; 61,000; 94,000) were solubilized by differential SDS-extraction and purified by prepara tive SDS-PAGE. The extracted proteins, reconstituted into lipid bilayer membranes, formed two types of pores with single-channel conductances of 2.2 nS (pore diameter of 1.4 nm) and 0.3 nS (pore diameter not determined), respectively. Carotenoids and lipopolysaccharide were found to be associated with the extracted major proteins. | | | |
Reference
| Z. Naturforsch. 44c, 165—169 (1989); received October 10 1988 | | | |
Published
| 1989 | | | |
Keywords
| Carotenoid-Binding Protein, Cyanobacterium, Outer Membrane Protein, Porin, Protease-Resist ance, Synechocystis | | | |
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| default:Reihe_C/44/ZNC-1989-44c-0165.pdf | | | | Identifier
| ZNC-1989-44c-0165 | | | | Volume
| 44 | |
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