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'Outer Membrane Protein' in keywords Facet   Publication Year 1989  [X]
Facet   section ZfN Section C  [X]
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1Author    UweJ. Jürgens, Roland BenzRequires cookie*
 Title    Pore-Forming Activity of Outer Membrane Extracts from the Unicellular Cyanobacterium Synechocystis sp. PCC 6714  
 Abstract    Cell walls of the unicellular cyanobacterium Synechocystis sp. PCC 6714, isolated from cell homogenates, were found to be unusually resistant against extraction with various detergents, organic solvents, chaotropic agents, and proteases. The major outer membrane proteins (M r 67,000; 61,000; 94,000) were solubilized by differential SDS-extraction and purified by prepara­ tive SDS-PAGE. The extracted proteins, reconstituted into lipid bilayer membranes, formed two types of pores with single-channel conductances of 2.2 nS (pore diameter of 1.4 nm) and 0.3 nS (pore diameter not determined), respectively. Carotenoids and lipopolysaccharide were found to be associated with the extracted major proteins. 
  Reference    Z. Naturforsch. 44c, 165—169 (1989); received October 10 1988 
  Published    1989 
  Keywords    Carotenoid-Binding Protein, Cyanobacterium, Outer Membrane Protein, Porin, Protease-Resist­ ance, Synechocystis 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0165.pdf 
 Identifier    ZNC-1989-44c-0165 
 Volume    44