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1997 (1)
1Author    W. Nikolaus Kiihn-VeltenRequires cookie*
 Title    Effects of Compatible Solutes on Mammalian Cytochrome P450 Stability  
 Abstract    The sensitivity of pig cytochrome P450cl7 (CYP17), an endoplasmic reticulum membrane-bound enzyme, towards heat denaturation (48 °C) was measured by the P450-to-P420 spectral transition indicating conforma­ tional labilization of the protein. Both sucrose and glu­ cose have comparable and increasingly protective effects at concentrations ranging from 100 to 800 mM, while ec­ toine, a novel zwitterionic compatible solute which regu­ lates bacterial osmoadaptation and stabilizes cyto­ plasmic enzymes, has a strong labilizing effect on CYP17 and favours its proteolytic inactivation possibly by elec­ trostatic derangements. Sucrose or glucose, but not ec­ toine, can therefore eventually be proposed as compati­ ble stabilizers of cytochrome P450 structures. 
  Reference    Z. Naturforsch. 52c, 132—135 (1997); received September 18/November 18 1996 
  Published    1997 
  Keywords    Cytochrome P450 Protein Stability, Osmolytes, Ectoine, Sucrose 
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 TEI-XML for    default:Reihe_C/52/ZNC-1997-52c-0132_n.pdf 
 Identifier    ZNC-1997-52c-0132_n 
 Volume    52