| | 1 | Author
| Dietrich Werner, W. Alburga, D. Ittrich, H. Eidem, ArieT. Hierfelder | Requires cookie* | | | Title
| Malonate and Krebs Cycle Intermediates Utilization in the Presence of other Carbon Sources by Rhizobium japonicum and Soybean Bacteroids  | | | | Abstract
| Free living cells of Rhizobium japonicum 61-A-101 and bacteroids from Glycine max var. Mandarin infected with the same strain utilized malonate with a substrate saturation greater than 10~2 mol/1. At low concentrations of malonate (10~5 mol/1) the free living cells were significantly more active in utilizing malonate than bacteroids. In bacteroids two substrate saturation ranges were found, one between 3 x l 0 _4m ol/l and 10-3 mol/1, the other at more than 10-2 mol/1. Utilization of malonate was not affected by 10 to 100 times larger concentrations o f either arabinose or xylose. IO-3 mol/1 succinate inhibited the utilization o f malonate (10-4 mol/1) completely in bacteroids, and by 90% in free living cells. Succinate utilization (10-4 mol/1) was reduced in those cells exposed to 100 times higher malonate concentration only by 20-30% . Utilization and incorporation o f pyruvate and 2-oxoglutarate into bacteroids was also only slightly affected by 100 times larger malonate concentration. Citrate utilization by bacteroids however was reduced by more than 70%. The rate o f endoxidation o f malonate as sole carbon source (14C 0 2 production from [2-14C] malonic acid)was about 1.5 pmol • h-1 • mg protein-1 and about half the rate with Pseudomonas putida and 70% o f the rate with Pseudomonas fluorescens under the same conditions (pH 6.0, 28 °C). | | | |
Reference
| Z. Naturforsch. 37c, 921 (1982); received May 19 1982 | | | |
Published
| 1982 | | | |
Keywords
| Rhizobium japonicum, Rhizobium Bacteroids, Malonate Utilization, Organic Acids Utilization | | | |
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| default:Reihe_C/37/ZNC-1982-37c-0921.pdf | | | | Identifier
| ZNC-1982-37c-0921 | | | | Volume
| 37 | |
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