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1983 (1)
1979 (1)
1Author    HansWerner Miiller, Margareta BaltscheffskyRequires cookie*
 Title    On the Oligomycin-Sensitivity and Subunit Composition of the ATPase Complex from Rhodospirillum rubrum  
 Abstract    Two alternative procedures for isolation of the oligomycin-sensitive ATPase complex (E. C. 3.6.1.3) from R. rubrum chromatophores are compared with respect to ease, rapidity, and yield. The inhibitory effect of oligomycin on the membrane-bound Ca2+ -ATPase activity is increased during storage of the chromatophores, whereas the effect of oligomycin on the membrane-bound Mg2+ -ATPase activity does not change within a week. Oligomycin-sensitivity of the solubilized ATPase complex depends on the isolation procedure. The enzyme complex consists of at least nine different polypeptides with the apparent molec­ ular weights of (. The polypeptides 2 — 4, 7, and 8 represent subunits of coupling factor 1. 
  Reference    Z. Naturforsch. 34c, 229 (1979); received January 4 1979 
  Published    1979 
  Keywords    Rhodospirillum rubrum, ATPase, Subunits, Oligomycin 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0229.pdf 
 Identifier    ZNC-1979-34c-0229 
 Volume    34 
2Author    W. Erner Kroll, M. Onika Löffler, F. Rie, H. Elm, S. ChneiderRequires cookie*
 Title    Energy Parameters, Macromolecular Synthesis and Cell Cycle Progression of in vitro Grown Ehrlich Ascites Tumor Cells after Inhibition of Oxydative ATP Synthesis by Oligomycin  
 Abstract    1. In order to elucidate the significance of oxidative ATP production for the proliferation of Ehrlich ascites tum or cells, cell cycle progression, energy m etabolism and m acromolecular synthesis in the presence of oligomycin were studied. 2. In the presence of the inhibitor (20 ng/m l), lactate production and glucose uptake of the cells increased by about 30 — 35% as com pared to controls; oxygen consum ption was maximally inhibited by 30-45% and could not further be reduced by higher concentrations o f the inhibitor. ATP/ADP ratios of the oligomycin treated cells and control cells were not significantly different. 3. In the first passage in the presence o f oligomycin proliferation o f the cells is reduced to about 50% that of controls; without severely affecting viability (dye exclusion test). In the second passage with oligomycin cell proliferation completely arrests. As was shown by flow cytometric analysis and BrdU-H33258 technique of flow cytometry, cells accum ulate in the early S phase; division of cells which are in the S-and G 2 M com partm ent at the beginning o f oligomycin treatment accounts for the increase of cell num ber in the first passage in the presence of oligomycin. On recultivation in the third passage in the absence of the inhibitor cells take up proliferation again; an increase of cell num ber o f about 60% o f controls was observed within 24 h. 4. In the presence o f oligomycin incorporation of [2-14C ]thym idine is reduced to about 20% of the controls within 8 h, incorporation of [U -l4C]lysine begins to slow down im m ediately after treatment with the inhibitor, the same is true for the incorporation o f [2-14C]uridine. Transport of a-aminoisobutyric acid into the cells is not severely affected. 5. It is suggested, that not lack of energy by inhibition o f oxidative phosphorylation accounts for the arrest of cell cycle progression in the presence o f oligomycin but rather the blockade of transport of cytoplasmatic (glycolytic) ATP into m itochondria, which is caused by the high potential built up across the m itochondrial m em brane in the presence o f this inhibitor. 
  Reference    Z. Naturforsch. 38c, 604 (1983); received March 17 1983 
  Published    1983 
  Keywords    Ehrlich Ascites Tumor Cells, Oligomycin, Energy M etabolism, Cell Cycle Progression 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0604.pdf 
 Identifier    ZNC-1983-38c-0604 
 Volume    38