| | 1 | Author
| SatishK. Sharma, JinnieM. Garrett, StewartA. Brown | Requires cookie* | | | Title
| Separation of the S-Adenosylmethionine: 5-and 8-Hydroxyfuranocoumarin O-Methyltransferases of Ruta graveolens L. by General Ligand Affinity Chromatography  | | | | Abstract
| Two S-adenosyl-L-methionine:furanocoumarin O-methyltransferases of R . graveolens, acting at the 5-and 8-hydroxyls of the psoralen nucleus, were completely resolved by adsorption on a general affinity ligand, 5 -(3-carboxypropanamido) xanthotoxin, followed by specific desorption by bergaptol and xanthotoxol, respectively. The 5-O-methyltransferase was purified 450-fold by this procedure, the 8-O-methyltransferase 112-fold, and both enzyme fractions were electrophoretically homogeneous. No resolution could be achieved of the activity against two 5-hydroxypsoralens or of the activity against two 8-hydroxypsoralens, and conclusive evidence is presented for the existence of only one 5-O-methyltransferase and only one 8-O-methyltransferase acting on linear furanocoumarins. In tro d u c tio n | | | |
Reference
| Z. Naturforsch. 34c, 387 (1979); received March 9 1979 | | | |
Published
| 1979 | | | |
Keywords
| Coumarins, Furanocoumarins, O-Methyltransferases, Affinity Chromatography, R u ta graveolens | | | |
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| default:Reihe_C/34/ZNC-1979-34c-0387.pdf | | | | Identifier
| ZNC-1979-34c-0387 | | | | Volume
| 34 | |
| 2 | Author
| KarlDietrich Hauffe, KlausH. Ahlbrock, D. Ierk Scheel | Requires cookie* | | | Title
| Elicitor-Stimulated Furanocoumarin Biosynthesis in Cultured Parsley Cells: S-Adenosyl-L-Methionine:BergaptoI and S-Adenosyl-L-Methionine: Xanthotoxol O-Methyltransferases | | | | Abstract
| Cultured parsley cells (Petroselinum crispum) treated with an elicitor from the soybean patho gen, P hytophthora m egasperm a f. sp. glycinea, were used as source for the separation, extensive purification and characterization of S-adenosyl-L-methionine: xanthotoxol and S-adenosyl-L-m ethionine: bergaptol O-methyltransferases. The products, xanthotoxin and bcrgapten, were among the most abundant coumarin derivatives accumulated in the culture fluid of elicitor-treated parsley cells. The latter enzyme also catalyzed the methylation of 5-hydroxyxanthotoxin to isopimpinellin, another major coumarin derivative in this system. The activities of both enzymes showed transient increases upon elicitor application prior to the accumulation of the products. No activity was detectable in untreated cells. Activity increases of the O-methyltransferases occurred a few hours later than those of phenylalanine ammonia-lyase and 4-coumarate:CoA ligase. | | | |
Reference
| Z. Naturforsch. 41c, 228 (1986); received October 14. 1985 | | | |
Published
| 1986 | | | |
Keywords
| birthday Furanocoumarins, Phytoalexins, O-Methyltransferases, Fungal Elicitor, Cell Suspension Cultures | | | |
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| default:Reihe_C/41/ZNC-1986-41c-0228.pdf | | | | Identifier
| ZNC-1986-41c-0228 | | | | Volume
| 41 | |
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