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'Nucleosomes' in keywords
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1981 (1)
1978 (1)
1Author    Jürgen Bode, Ulridi-Dieter StandtRequires cookie*
 Title    Structural Aspects of Histone Complexes and Nucleosomes Revealed by the Accessibility of Cysteine Side Chains  
 Abstract    Chemical modification with 5,5'-dithiobis-(2-nitrobenzoic acid) shows that histone H3 cysteines 110 (chicken) or 96 and 110 (calf) are com pletely protected in native chromatin and core particles but become unm asked sim ultaneously during a salt induced dissociation. In whole histone extracted from chicken erythrocyte chromatin, H3 Cys-110 residues experience a uniform environ­ m ent at 2 m NaCl and pH 5.5. In whole histone extracted under the same conditions from calf thymus, H3 Cys-96 provides a more accessible thiol group than Cys-110. At higher pH values a conform ational heterogeneity is induced causing a p artial exposure of both cysteine side chains. The kinetic approach described in the present work provides a highly sensitive means to probe the homogeneity of H3 containing protein complexes. 
  Reference    Z. Naturforsch. 33c, 884 (1978); received August 2 1978 
  Published    1978 
  Keywords    Histone, Chrom atin, Protein SH-Reactivity, Nucleosomes 
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 TEI-XML for    default:Reihe_C/33/ZNC-1978-33c-0884.pdf 
 Identifier    ZNC-1978-33c-0884 
 Volume    33 
2Author    Ruth Marx, Detlef DoeneckeRequires cookie*
 Title    Binding of Polylysine and Ethidium Bromide to Nucleosomal DNA: Comparison of Biochemical and Electron Microscopical Results  
 Abstract    Ethidium bromide and polylysine interact with nucleosomal D NA and lead to changes o f biochemical properties and to morphological changes as to the distance between the two core particles of a nucleosome dimer. With increasing polylysine concentration, the buoyant density of nucleosomes decreases and the accessibility of the nucleosomal DNA to micrococcal nuclease is lowered. Electron microscopy of polylysine treated nucleosome dimers reveals a shortening of the intemucleosomal distance as compared with controls. Treatment of nucleosomes with ethidium bromide leads to an enhanced accessibility of the nucleosomal DNA to micrococcal nuclease. Electron microscopy reveals an increase in length of the DNA connecting the two nucleosome cores in the presence o f the dye. Both the binding of polylysine and the treatment with ethidium bromide apparently do not affect the histone arrangement within the nucleosome core as suggested by chemical cross-linking o f histones and DNA with formaldehyde, and no obvious morphological changes of the nucleosome cores can be observed. 
  Reference    Z. Naturforsch. 36c, 149—156 (1981); received September 1 1980 
  Published    1981 
  Keywords    Nucleosomes, Polylysine, Ethidium Bromide, Biochemistry, Morphology 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0149.pdf 
 Identifier    ZNC-1981-36c-0149 
 Volume    36