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1994 (1)
1Author    ThiloC. Fischer, Sabine Groner, Ulrike Zentgraf, Vera HemlebenRequires cookie*
 Title    Evidence for Nucleosomal Phasing and a Novel Protein Specifically Binding to Cucumber Satellite DNA  
 Abstract    The nucleosomal organization and the protein-binding capability of highly repeated and methylated satellite DNA of cucumber (Cucumis sativus L.), comprising approx. 30% of the genome, were analyzed. Nucleosomal core DNA from satellite type I was prepared after micrococcal nuclease digestion of chromatin and sequenced. Most of the core sequences ob­ tained could be grouped in two main (A and B) and two minor groups (C and D) indicating a specific and complex phasing of nucleosomes on this satellite DNA. In vitro, gel retarda­ tion assays with cloned satellite DNA repeats (types I-IV) demonstrated a specific binding of nuclear proteins. These specific binding effects are also obtained with genomic, in vivo methylated and sequence heterogeneous (1 to 10% diversity) satellite type I DNA. For the first time in plants, a satellite DNA-binding protein with an apparent molecular weight of 14 kDa (SAT 14) was identified. 
  Reference    Z. Naturforsch. 49c, 79—86 (1994); received October 28/November 121993 
  Published    1994 
  Keywords    Cucumis sativus, DNA Methylation, DNA-Protein Interaction, Nucleosomal Phasing, Satellite DNA 
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 TEI-XML for    default:Reihe_C/49/ZNC-1994-49c-0079.pdf 
 Identifier    ZNC-1994-49c-0079 
 Volume    49