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1990 (1)
1981 (1)
1975 (1)
1Author    N. G. FazleyevRequires cookie*
 Title    Theoretical Study of the Indirect Quadrupole-Quadrupole Interactions in Metals and Alloys  
 Abstract    The indirect multipole interactions of nuclei and impurity paramagnetic ions in metals and alloys via conduction electrons are investigated by means of the dielectric function method. The Hamilto-nian of the indirect quadrupole-quadrupole interaction of impurity paramagnetic ions and nuclei is constructed selfconsistently, taking into account the exchange interactions and correlations of the conduction electrons as well as the antishielding effects. It is shown that the energy of these indirect quadrupole interactions of the nuclei and the paramagnetic ions decreases with the distance as R ~ 3 , oscillating with a period which is determined by the wave vector on the Fermi surface and the distance R. The influence of these indirect quadrupole-quadrupole interactions on the width and shape of the NMR lines is studied. 
  Reference    Z. Naturforsch. 45a, 380—384 (1990); received August 23 1989 in revised form December 1 1989 
  Published    1990 
  Keywords    Quadrupole, Interactions, Nuclei, Metals, Resonance 
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 TEI-XML for    default:Reihe_A/45/ZNA-1990-45a-0380.pdf 
 Identifier    ZNA-1990-45a-0380 
 Volume    45 
2Author    Margarethe Spindler-Barth, Ursula Bassemir, Paul Kuppert, Klaus-Dieter SpindlerRequires cookie*
 Title    Isolation of Nuclei from Crayfish Tissues and Demonstration of Nuclear Ecdysteroid Receptors  
 Abstract    Nuclei from hypodermis and midgut gland of the crayfish, Orconectes limosus, have been isolated with a slightly modified Chauveau-method. The isolated nuclei are well preserved and free from cytoplasmic contamination. They are able to incorporate [3H]UTP into acid-insoluble material. This labelling is a-amanitin sensitive. The isolated nuclei are devoid o f the mito­ chondrial/microsomal ecdysone-C-20-hydroxylase. The DNA: RNA: protein ratio is 1:0.8: 3.2 (using Triton X-100 during the isolation procedure the ratio is 1:0.4: 4.6) for nuclei from the hypodermis and 1:1: 3.8 for nuclei from the midgut gland. The absolute amount of DNA per cell is 1.6, resp. 3.5 pg. The final DNA-recovery is about 20%. The yield increases to about 60% if the final ultrazentrifugation step is omitted. The isolated nuclei bind 3H-ecdysone and 3H-20-OH-ecdysone specifically. As revealed by Scatchard analysis the affinity of the nuclear 20-OH-ecdysone receptor is 1 — 2 x 10~9 M. 
  Reference    Z. Naturforsch. 36c, 326 (1981); received October 16/November 17 1980 
  Published    1981 
  Keywords    Nuclei, Crayfishes, Nuclear Ecdysteroid Receptors 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0326.pdf 
 Identifier    ZNC-1981-36c-0326 
 Volume    36 
3Author    Hans-Ullrich Gebauer, Ursula Seitz, Ulrich SeitzRequires cookie*
 Title    Transport and Processing of Ribosomal R N A in Plant Cells after Treatment with Cycloheximide  
 Abstract    In freely suspended cells of parsley (P etroselin u m crispu m) protein synthesis is inhibited nearly totally by 5 /ug/m l cycloheximide within 10 min. This very low dose has a slight effect on the rRNA synthesis too. During an incubation period of 60 min with [32P] orthophosphate in the presence of the inhibitor (5 /ugjml) at least 65 — 70% mature rRNA are synthesized compared with the control. After 120 min the synthesis is progressively reduced to 60 — 65%, and after 240 min to 30-40% . Cycloheximide causes a delay of the processing of the precursor RNA species leading to an accumulation of these components. In addition to the 2.3 x 10® daltons RNA, normally detectable in pulse experiments, two further precursor molecules do emerge which under normal circumstances apparently are shortlived. Their molecular weights are 2.0 x 10® and 0.9 x 10® daltons. The pulse-chase technique and cell fractionation into nuclear and ribosomal parts enables us to demonstrate a rapid transfer of labelled 18S and 25S RNA during a 15 min chase treatment to the mature cytoplasmic ribosomes. Under these conditions no differential transport of the two com­ ponents takes place. The cells possess a pool of proteins. Therefore the formation of RNP-particles or ribosomes respectively and the transport from the nucleus to the cytoplasm are independent from protein synthesis for some time. 
  Reference    (Z. Naturforsch. 30c, 213 [1975]; received November 25 1974) 
  Published    1975 
  Keywords    Ribosomal RNA, Nuclei, Ribosomes, Cycloheximide, Plant Cell Culture 
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 TEI-XML for    default:Reihe_C/30/ZNC-1975-30c-0213.pdf 
 Identifier    ZNC-1975-30c-0213 
 Volume    30