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1Author    Anat Rozen, Mordechay Schönfeld, Elisha Tel-OrRequires cookie*
 Title    Fructose-Enhanced Development and Growth of the N 2 -Fixing Cyanobiont Anabaena azollae  
 Abstract    Fructose supported the heterotrophic growth of the cyanobiont Anabaena azollae, isolated from the water fern Azolla filiculoides, and also enhanced its growth in the light by 2-3-fold. Fructose was taken up at a high rate in the light and in the dark, in an energy-dependent reaction. The photosynthetic and respiratory activities of the fructose grown cells were modified: 0 2 evolu-tion in vivo was decreased by 40%, while PS I activity and dark respiration were 2-3-fold higher than in autotrophically grown cells. These changes were accompanied by 2-3-fold increase in heterocyst differentiation and by a 4-fold stimulation of nitrogenase activity. 
  Reference    Z. Naturforsch. 43c, 408—412 (1988); received February 1 1988 
  Published    1988 
  Keywords    Nitrogen Fixation, Symbiotic Cyanobacteria, Photosynthesis, Respiration, Heterotrophic Growth 
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 TEI-XML for    default:Reihe_C/43/ZNC-1988-43c-0408.pdf 
 Identifier    ZNC-1988-43c-0408 
 Volume    43 
2Author    KlausP. Bader, Anja RöbenRequires cookie*
 Title    Mass Spectrometric Detection and Analysis of Nitrogen Fixation in Oscillatoria chalybea  
 Abstract    By means of mass spectroscopic m easurements in an artifical gas atmosphere containing the stable nitrogen isotope 15N2 we were able to demonstrate nitrogen fixation capacity in the filamentous cyanobacterium Oscillatoria chalybea. Our technique proved to be well-suited also for investigations on the light-induced nitrogen fixation in the purple bacteria Rhodobacter sphaeroides and Rhodobacter capsulatus. Oscillatoria chalybea grown without combined nitrogen showed a substantial 15N2-uptake which could clearly be correlated with nitrogen fixation. Nitrate grown cultures did not show this nitrogen uptake or only to a minimal extent. Addition of ammonium chloride resulted in a rapid deactivation of the nitro-genase system. Similar observations have been made with other so-called switch-off effectors like phenazine methosulfate. The structural integrity of the filaments appeared to be a pre­ requisite for nitrogen fixation also in this organism, as even mild mechanical homogenization strongly inhibited the N2-uptake signals. Illumination of the assays under conditions where the photooxidition of water is not operational (Bader, K. P. (1994), Biochim. Biophys. A cta 1188, 2 1 3 -2 1 9) did not affect the nitrogen fixation in Oscillatoria chalybea. Illumination of cultures with concom itant release of oxygen from the w ater splitting reaction resulted in strong inhibition of 15N2-uptake. 
  Reference    Z. Naturforsch. 50c, 199 (1995); received November 11 1994/January 30 1995 
  Published    1995 
  Keywords    Mass Spectrometry, Nitrogen Fixation, Photosynthesis, Cyanobacterium 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0199.pdf 
 Identifier    ZNC-1995-50c-0199 
 Volume    50 
3Author    H. Erm, Ann Bothe, Gislene Barbosa, JohannaD.Requires cookie*
 Title    Nitrogen Fixation and Nitrate Respiration by A zospirillum brasilense  
 Abstract    Azospirillum The 0 2-sensitivitiy of N 2-fixation by the carotenoid forming strain Azospirillum brasilense Cd and the colourless strain Sp 7 is com pared in the present communication. As no difference in the reaction is observed with both strains, it is concluded that carotenoids do not protect nitrogenase from damage by 0 2. Azospirillum spp. have also been shown to perform N O '-dependent ^ -f i x ­ ation. The physiological properties of this reaction are described in more detail in the present communication. Evidence is presented that NO^-dependent N 2-fixation is a transitory reaction, proceeding only as long as the enzymes o f assimilatory nitrate reduction are synthesized by the cells. 
  Reference    Z. Naturforsch. 38c, 571—577 (1983); received March 31 1983 
  Published    1983 
  Keywords    Nitrogen Fixation, N itrate Respiration, Denitrification, Nitrogenase Protection, Carotenoids 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0571.pdf 
 Identifier    ZNC-1983-38c-0571 
 Volume    38 
4Author    DietrichW. Erner, RobertB M Ellor, MichaelG H Ahn, Hans GrisebachRequires cookie*
 Title    Soybean Root Response to Symbiotic Infection Glyceollin I Accumulation in an Ineffective Type of Soybean Nodules with an Early Loss of the Peribacteroid Membrane  
 Abstract    A glyceollin I accumulation o f about 6000 pm ol • mg dry weight-, a tenfold increase above control root tissue, was found in one type o f nodule from Glycine m ax which had been infected with a fix ~ strain (61-A-24) o f Rhizobium japonicum . In nodules infected with one other ineffective (ßx~) strain o f Rhizobium japonicum (RH 31-Marburg) or with two f i x + strains o f Rhizobium japonicum (61 -A -101 and U SD A 110) no increase in glyceollin I concentrations above control values was found at either 20 d or 34 d after infection. N odules infected with Rhizobium japonicum 61-A-24 are distinguished by an early loss o f the peribacteroid membrane in the infected host cell, whilst the bacteroids them selves remain stable. 
  Reference    Z. Naturforsch. 40c, 179—181 (1985); received N ovem ber 23 1984 
  Published    1985 
  Keywords    Glyceollin, Glycine max, Nitrogen Fixation, N odules, Peribacteroid Membrane 
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 TEI-XML for    default:Reihe_C/40/ZNC-1985-40c-0179.pdf 
 Identifier    ZNC-1985-40c-0179 
 Volume    40 
5Author    Karin Kloos, UrsulaM. Hiisgen, Hermann BotheRequires cookie*
 Title    DNA-Probing for Genes Coding for Denitrification, N2-Fixation and Nitrification in Bacteria Isolated from Different Soils  
 Abstract    Bacteria isolated from different layers of four soils of the Cologne area were analyzed for denitrifying, nitrifying and N2-fixing isolates by colony hybridization using gene probes. In the soils tested, the percentage of denitrifying bacteria among the total population isolated was 3 -8 % (in one case exceptionally 15%) and thus small. Denitrifying bacteria were partic­ ularly enriched in the upper layer (depth ~ 5 cm) and were present only in low amounts at 25 cm depth in two gleysol soils. Nitrate apparently did not determine the distribution of denitrifying bacteria in these soils. The potential denitrification activity of different soil layers coincided with the distribution pattern of isolates assessed by DNA-probing. The total number of bacteria and of denitrifying isolates was considerably higher in or at the roots of plants than in the bulk, root-free soil adjacent to the plants. The percentage of the isolated aerobic N2-fixing bacteria varied between 0 -3 % , and these bacteria could be isolated mainly from the upper 5 cm layer. A small portion of the isolates hybridized with the probe coding for part of one subunit of ammonia monooxygenase from Nitrosomonas europaea. The inves­ tigation showed that DNA-probing can provide useful information about the relative distri­ bution of denitrifying and N2-fixing bacteria in different soils and their layers. 
  Reference    Z. Naturforsch. 53c, 69—8 (1998); received October 14/November 6 1997 
  Published    1998 
  Keywords    DNA-Probing, Colony Hybridizations, Soil Ecology, Denitrification, Nitrification, Nitrogen Fixation 
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 TEI-XML for    default:Reihe_C/53/ZNC-1998-53c-0069.pdf 
 Identifier    ZNC-1998-53c-0069 
 Volume    53 
6Author    Kassem Alef, Hans-Joachim Burkardt, Hans-Joachim Horstmann, WalterG. ZumftRequires cookie*
 Title    Molecular Characterization of Glutamine Synthetase from the Nitrogen-Fixing Phototrophic Bacterium Rhodopseudomonas palustris1  
 Abstract    The phototrophic bacterium Rhodopseudomonas palustris assimilated ammonium via glutamine synthetase and glutamate synthase. Diazotrophic and ammonium-grown cells had high levels of both enzymes, whereas enzymes o f alternative assimilatory pathways were absent or had only low activities. Glutamine synthetase was purified to electrophoretic homogeneity within three steps by dye-ligand and ion exchange chromatography. Electron microscopy revealed a dodecameric molecular entity which was in accordance with parameters derived from electrophoretic techniques. The molecular weight of the enzyme monomer was 55800; that o f the dodecamer 670000. The amino acid composition o f R. palustris glutamine synthetase was determined and compared by a statistical method with other known enzyme compositions from prokaryotic and eukaryotic origins. 
  Reference    Z. Naturforsch. 36c, 246—254 (1981); received December 231980 
  Published    1981 
  Keywords    Rhodopseudomonas palustris, Glutamine Synthetase, Dye-Ligand Chromatography, Nitrogen Fixation, Ammonium Assimilation 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0246.pdf 
 Identifier    ZNC-1981-36c-0246 
 Volume    36