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1980 (1)
1Author    Renate Horcher, John Wilcockson, D. Ietrich, W. ErnerRequires cookie*
 Title    Screening for Mutants of Rhizobium japonicum with Defects in Nitrogen Fixing Ability  
 Abstract    Mutants o f Rhizobium japonicum with reduced ex planta nitrogenase activity could be isolated with high frequency by direct screening of ultra-violet mutagenized bacteria growing as spots on the surface o f an appropriate agar medium permitting derepression of nitrogenase synthesis. Small glass chambers fitted with a serum cap were pushed into the agar around each spot of growth, forming a small enclosed gas space which was made to 10% acetylene, permitting assessment o f nitrogenase activity by the acetylene reduction test. Four mutants were isolated from a total of 305 screened spots. Two mutants had almost no ex planta activity, one o f which had no symbiotic activity despite normal nodulation (ineffective), the other had only somewhat reduced activity symbiotically. Two other mutants with less than half wild-type activity ex planta were normal in symbiosis. 
  Reference    Z. Naturforsch. 35c, 729—732 (1980); received May 30 1980 
  Published    1980 
  Keywords    Rhizobium japonicum, Nitrogenase, Nif-Mutants, Ineffective Nodules 
  Similar Items    Find
 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0729.pdf 
 Identifier    ZNC-1980-35c-0729 
 Volume    35