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'Nicotiana tabacum' in keywords
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1Author    Jochen Berlin, Ludger WitteRequires cookie*
 Title    Effects of Glyphosate on Shikimic Acid Accumulation in Tobacco Cell Cultures with Low and High Yields of Cinnamoyl Putrescines  
 Abstract    To study the flow of carbon through the shikimate pathway in tobacco cell cultures with low and high yields o f cinnamoyl putrescines, the cell cultures were treated with glyphosate. In the presence o f glyphosate the levels o f free shikimic acid were increased more than 300-fold by both cell lines. Despite of a normally 10-fold higher level o f cinnamoyl putrescines, the high yielding cell line accumulated only 25% more free shikimic acid than the low yielding cell line. This result together with earlier observations indicated that the increased formation o f cinnamoyl putrescines was rather limited by the activity o f phenylalanine ammonia lyase than by increased substrate supply caused by alterations in the shikimate pathway. 
  Reference    Z. Naturforsch. 36c, 210 (1981); received November 27 1980 
  Published    1981 
  Keywords    Nicotiana tabacum, Suspension Cultures, Shikimic Acid, Cinnamoyl Putrescines, Glyphosate 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0210.pdf 
 Identifier    ZNC-1981-36c-0210 
 Volume    36 
2Author    Ursula Bruemmer, Christian Paulsen, G. Unda Spremberg, Friedlieb Seehofer, Volker Heemann, B.A T Cigaretten-Fabriken, ForschungUnd Gmbh, Entwicklung, Volker SinnwellRequires cookie*
 Title    New Cembranoids from Burley T ob acco  
 Abstract    The liquid C 0 2-extract of aged burley tobacco was investigated for its content of diterpenoids, which are considered as im portant precursors of flavour constituents. Among several cembranoids, already known from various tobacco types, 4,8-dimethyl-8-hydroxy-l l-(l-methyl-ethyl)-14-oxo-pentadeca-5,9-diene-4-olide, a new natural product, could be identified, evidently originating from 2,6,11-cem-bratriene-4,8-diol by oxidative cleavage of the 11,12-double bond with subsequent cyclization to the seco-lactone 14. 
  Reference    Z. Naturforsch. 36c, 1077—1080 (1981); received July 301981 
  Published    1981 
  Keywords    Nicotiana tabacum, Solanaceae, Burley Tobacco, Diterpenoids, Cembranoids, seco-Lactone 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-1077_n.pdf 
 Identifier    ZNC-1981-36c-1077_n 
 Volume    36 
3Author    G. H. Schmida, A. R. Adunz3, K.P B Ader3, B. Myśliwa-Kurdzielb, K. Strzał, J. KrukbRequires cookie*
 Title    Action of an Antiserum to a-Tocoquinone on Photosystem II-Particle Preparations of N icotiana tabacum  
 Abstract    An antiserum to a-tocoquinone was prepared by immunization of rabbits. Immunization was obtained by injection of a conjugate consisting of the hapten a-tocoquinone attached to methylated ovalbumin into the rabbit. The antiserum recognizes the 3,4-dimethyl-p-benzo-quinone group of the m olecule as well as part of the immediate vicinity to the side chain. This is concluded from the fact that the antibody has som e affinity also to plastoquinone. No reaction of the antibody is observed with a-tocopherol hydroquinone or a-tocopherol. Reac­ tion of the antiserum to a-tocoquinone with photosystem Il-particle preparations from to­ bacco affects the functionality of the preparation. Chlorophylla-fluorescence emission is quenched without an alteration of the em ission spectrum. Concomitant with this fluorescence quenching, the lifetime of two fluorescence com ponents namely that of a fast and a slower component are shortened. By analogy with the literature the fast component is associated with chlorophylla of the reaction center core and that of the slow com ponent with the antenna system in which the lifetime parameter is shortened by the antibody from 3.42 ns to 1.795 ns. The action on the fast component is less and leads to a shortening of the lifetime parame­ ter from 0.373 ns to only 0.249 ns. The effect is interpreted in terms of an enhancement of linear photosynthetic electron transport possibly due to an inhibition of the cyclic electron transport around PS II, discovered by Gruszecki et al. 
  Reference    Z. Naturforsch. 51c, 691—6 (1996); received May 14/July 17 1996 
  Published    1996 
  Keywords    Antiserum, a-Tocoquinone, Photosystem II, Fluorescence Lifetime, Nicotiana tabacum 
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 TEI-XML for    default:Reihe_C/51/ZNC-1996-51c-0691.pdf 
 Identifier    ZNC-1996-51c-0691 
 Volume    51 
4Author    Jochen Berhn, Brigitte VollmerRequires cookie*
 Title    Effects of a-Aminooxy-ß-phenylpropionic Acid on Phenylalanine Metabolism in /r-Fluorophenylalanine Sensitive and Resistant Tobacco Cells  
  Reference    Z. Naturforsch. 34c, 770 (1979); received May 18 1979 
  Published    1979 
  Keywords    Nicotiana tabacum, Suspension Cultures, a-A m inooxy-ß-phenylpropionic Acid, Chorismate Mu-tase, Phenylalanine, Pool Sizes 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0770.pdf 
 Identifier    ZNC-1979-34c-0770 
 Volume    34 
5Author    Jochen Berlin, BerndM. Artin, Jerzy Nowak, LudgerW. Itte+, Victor Wray, D. Ieter Strack+, G. Esellschaft Für, Biotechnologische Forschung, BhM. Ascheroder, W. EgRequires cookie*
 Title    Effects of Permeabilization on the Biotransformation of Phenylalanine by Immobilized Tobacco Cell Cultures  
 Abstract    Im m obilized tobacco cells excreted only a small proportion of their main secondary m etabolite caffeoylputrescine into the culture m edium . Experim ents designed to release more o f the com ­ pound by perm eabilization caused the loss o f caffeoylputrescine, probably by oxidative reactions. M oreover, rather mild treatments with pejm eabilizing agents (e.g. «-propanol) resulted in severe growth inhibition. The ability of perm eabilized cells to form caffeoylputrescine and other hy-droxycinnam oyl conjugates from phenylalanine decreased considerably even when such cells were still able to m etabolize phenylalanine into various ethyl acetate extractable com pounds (e.g. hydroxycinnamic acids and acetophenones). The formation of new biotransformation products suggests that perm eabilized cells could be used as a tool for testing the enzymatic capabilities o f a cell culture. 
  Reference    Z. Naturforsch. 44c, 249 (1989); received D ecem ber 5 1988 
  Published    1989 
  Keywords    Nicotiana tabacum, Cell Suspension Cultures, Imm obilized Cells, Permeabilized Cells, Biotrans­ form ation, Phenylalanine 
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 TEI-XML for    default:Reihe_C/44/ZNC-1989-44c-0249.pdf 
 Identifier    ZNC-1989-44c-0249 
 Volume    44 
6Author    Josef Vinnemeier, Wolfgang Dröge-Laser, ElfriedeK. Pistorius, Inge BroerRequires cookie*
 Title    Purification and Partial Characterization of the Streptomyces viridochromogenes TÜ494 Phosphinothricin-N-acetyltransferase Mediating Resistance to the Herbicide Phosphinothricin in Transgenic Plants  
 Abstract    A purification scheme for the enzyme phosphinothricin-N-acetyltransferase (PAT) origi­ nating from Streptomyces viridochrom ogenes {pat-gene product from Streptom yces virido­ chromogenes) and mediating herbicide resistance to transgenic plants was developed. The enzyme was isolated from a transformed and overproducing Escherichia coli strain. With a combination of ammonium sulfate fractionation, chromatography on D EAE-Sephadex A50-, Phenylsepharose-, Hydroxylapatite-and FPLC-Superose 12-columns it was possible to obtain PAT which was at least 90 % homogeneous on the basis of SDS-PAGE. The properties of the isolated PAT were compared with the properties of PAT from S. hygroscopicus (bar-gene product from S. hygroscopicus) previously isolated and characterisized by Botterman, J., Gossele, V., Thoen, C., Lauwereys, M. (1991), G ene 102, 33-37. Differences were observed in the molecular masses of the two native enzymes (PAT from S. viridochrom ogenes being a dimer of 40 kD and PAT from S. hygroscopicus being a m onomer of 21 kD), and in the tem perature sensitivity of the two enzymes (the PAT from S. viridochrom ogenes being slightly more tem perature stable than PAT from S. hygroscopicus). However, since the pat and the bar-gene are to 85 % homologous, substantial similarities exist between the two enzymes especially in the kinetic values and the substrate specificity. The isolated S. virido­ chromogenes PAT did not acetylate putative substrates present in the plant cell. Antibodies were raised against the isolated protein. This antiserum was able to detect PAT in transgenic plants and therefore is suitable to analyse the fate of the protein in such plants under various stress conditions. 
  Reference    Z. Naturforsch. 50c, 796—805 (1995); received August 16 1995 
  Published    1995 
  Keywords    Biosafety, Herbicide Resistance, Nicotiana tabacum, Phosphinothricin-A'-acetyltransferase (PAT), Transgenic Plants 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0796.pdf 
 Identifier    ZNC-1995-50c-0796 
 Volume    50