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1980 (1)
1976 (1)
1Author    HansPeter Kulas, F.Alfred AndererRequires cookie*
 Title    Differences in the Lipid Distribution in Subcellular Fractions of Mouse Fibroblasts Derived from Logarithmic and Stationary G row th  
 Abstract    The lipid class compositions of subcellular fractions of SV40-transformed mouse fibroblasts derived from the logarithmic and stationary growth phase were compared. Cell populations of the stationary growth phase showed a relative decrease of the protein content and an increase of tri­ glycerides and alkoxydiglycerides which could be located in the non-sedimenting fraction and in the nuclei and mitochondria containing fraction, respectively. Furtheron, distinct shifts in the subcellular distribution of those lipid classes could be observed which exhibited no relative overall increase or decrease when the cells of both growth phases were compared. In the crude plasma membrane fraction the ratio "lipid class/protein" remained about constant with the exception of the phospholipids and alkoxydiglycerides. 
  Reference    (Z. Naturforsch. 31c, 179 [1976]; received October 27/November 20 1975) 
  Published    1976 
  Keywords    Lipid Distribution, Mouse Fibroblasts, Growth Curve, Subcellular Fractions 
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 TEI-XML for    default:Reihe_C/31/ZNC-1976-31c-0179.pdf 
 Identifier    ZNC-1976-31c-0179 
 Volume    31 
2Author    Volker Kinzel, James RichardsRequires cookie*
 Title    Culture Density and Age-Dependent Interactions of 3 T 3 and SV 3T 3 Cells with Immobilized and Soluble Lens Culinaris Lectin  
 Abstract    Short term binding of both Balb/c derived 3T 3 cells and SV 3T3 cells to 2B-Sepharose coated with Lens culinaris lectin (LCL) was compared with their LCL-induced agglutinability in relation to culture density and age. The cells were grown for 4 days either to low (LD) or to high density (HD); for certain experiments HD-cells were stimulated (HDS) by a short trypsin treatment and/ or by addition of fresh medium. HD-3T3 cells bound somewhat faster to immobilized LCL than LD -3T3 cells, although LD -3T3 cells agglutinated at lower LCL concentrations. In the case of SV 3T3 cells, binding was much less pronounced for HD than for LD cells. The agglutinability of HD-SV3T3 cells, however, was greater than that of the other transformed groups. Upon stimula­ tion, binding and agglutinability data of both HD cell lines began to resemble the results obtained with LD-cells. Taken together, the data revealed in all cases an inverse relationship between bind­ ing and agglutinability, i. e., high agglutinability was closely correlated with slower binding and vice versa. The results indicate that culture density and age-dependent differences in cell surface architecture can be detected by short term binding to immobilized LCL. 
  Reference    Z. Naturforsch. 35c, 284—2 (1980); received August 24/November 22 1979 
  Published    1980 
  Keywords    Mouse Fibroblasts, Density Dependence, Cell Surface, Immobilized Lectin, Lens Culinaris Lectin 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0284.pdf 
 Identifier    ZNC-1980-35c-0284 
 Volume    35