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'Methylene Blue' in keywords
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1984 (1)
1980 (1)
1Author    Norio Miyoshi, Giiti TomitaRequires cookie*
 Title    Solubilization of Methylene Blue in Reversed Micelles, Effect of Water  
 Abstract    The solubilization of methylene blue in dodecylammonium propionate reversed micelles in cyclohexane depended strongly on the solubilized water content. Methylene blue solubilized in the micelle of which head groups are not completely hydrated by bound water exhibited the new absorption bands at 495 and 270 nm, but these bands did not appear in the micelle containing free water. This dye had the absorption bands at 655 and 290 nm in the latter micelle. The solubilizing process and solubilized state of methylene blue in the reversed micelles were discussed with results obtained. 
  Reference    Z. Naturforsch. 35b, 741—745 (1980); received February 20 1980 
  Published    1980 
  Keywords    Methylene Blue, Reversed Micelles, Water Content, Solubilization 
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 TEI-XML for    default:Reihe_B/35/ZNB-1980-35b-0741.pdf 
 Identifier    ZNB-1980-35b-0741 
 Volume    35 
2Author    S. G. Mauriño, M. A. Vargas, C. Echevarría, P.J A Paricio, J.M M AldonadoRequires cookie*
 Title    Red-Light Effects Sensitized by Methylene Blue on Nitrate Reductase from Spinach (Spinacia oleracea L.) Leaves  
 Abstract    Nitrate reductase from spinach (Spinacia oleracea L.) leaves, w hich had been inactivated in vitro by incubation with N A D H and cyanide, was fully reactivated in m inutes when irradiated in anaerobic conditions with red light in the presence o f m ethylene blue. Both the rate and the extent o f reactivation increased with light intensity (6 to 100 W -m " 2) and dye concentration (1 to 10 jiM). On the contrary, photoreactivation was com pletely abolished when N A D H or ethylenediaminetetra-acetic acid were present during irradiation. W e propose that m ethylene blue, when photo excited, exhibits a redox potential positive enough to reoxidise the CN~-re-duced molybdenum complex settled in the inactive enzym e, thus causing its reactivation. On the other hand, prolonged irradiation o f nitrate reductase, under air and in the presence o f methylene blue, promoted an oxygen-dependent irreversible inactivation o f the two partial activities o f the enzyme. This inactivation was m arkedly enhanced in 77% deuterated water and greatly prevented by azide, which indicates that singlet oxygen is the species primarily involved in the photooxidative inactivation o f the enzym e. 
  Reference    Z. Naturforsch. 39c, 1079—1084 (1984); received July 27 1984 
  Published    1984 
  Keywords    Methylene Blue, Nitrate Reductase, Photosensitization, Singlet O xygen, Spinacia 
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 DEBUG INFO      
 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-1079.pdf 
 Identifier    ZNC-1984-39c-1079 
 Volume    39