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'Membrane Fusion' in keywords Facet   Publication Year 1984  [X]
Facet   section ZfN Section C  [X]
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1Author    Flavia Pasquali, G. Ianfranco, M. Enestrina, R. Enzo, A. NtoliniRequires cookie*
 Title    Fusion of Large Unilamellar Liposomes Containing Hemocyanin with Planar Bilayer Membranes  
 Abstract    Large unilamellar vesicles were prepared by detergent rem oval from micelles containing phosphatidylcholine, phosphatidylethanolam ine and phosphatidylserine. Liposomes were then interacted with Megathura crenulata hemocyanin, a well studied channel former. Incubation o f the resulting proteoliposomes on one side of a phosphatidylserine-containing planar bilayer under fusion conditions yielded strong current increases. Such increase is due to insertion o f ionic channels from the liposomes into the planar bilayer. Studying the effects o f Ba2+ on the electrical properties of the channel we could show that the protein is always inserted into a bilayer during this process, i.e. fusion of proteoliposomes with the artificial m em brane occurs. The strong non linearity of the current-voltage curve of the hemocyanin pore could be used as a probe o f the extent to which fusion preserves the orientation of the channel through the bilayer. 
  Reference    Z. Naturforsch. 39c, 147—155 (1984); received September 22 1983 
  Published    1984 
  Keywords    Membrane Fusion, Large Liposomes, Planar Bilayer, Hemocyanin Channel, Asymmetrical Insertion 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-0147.pdf 
 Identifier    ZNC-1984-39c-0147 
 Volume    39