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1995 (1)
1Author    JanuszVladimír Blasiaka, Zofia Kleinwächterb, RenataŽ. Walter3, AludovábRequires cookie*
 Title    Interaction of Organophosphorus Insecticide Methylparathion with Calf Thymus D N A and a Synthetic D N A Duplex  
 Abstract    The interaction of an organophosphorus insecticide m ethylparathion (O.O-dimethyl 0-4-nitrophenyl phosphorothioate) with double-stranded DNA was characterized by UV and circular dichroism (CD) spectroscopy. Two kinds of DNA were employed: calf thymus DNA (CT DNA) and a synthetic two-stranded oligomer of sequence 5'-d(TTG GA TCCG AA TT-C A A G CTT)-3\ Melting curves and CD spectra were taken for the DNAs in the presence of the insecticide at methylparathion/DNA base pair molar ratio of 0.5. The insecticide evoked a decrease of the melting tem perature and a broadening of the transition range for CT DNA. Similar effects were observed for the synthetic oligomer but they were less pronounced than in the case of CT DNA. M ethylparathion evoked a slight shift and an increase in the ampli­ tude of the negative band in the CD spectra of both DNAs. O btained results indicate that methylparathion may perturb the thermal stability and conformation of DNA, which is an evidence that the insecticide has an ability to interact directly with DNA. 
  Reference    Z. Naturforsch. 50c, 820—823 (1995); received August 8/Septem ber 19. 1995 
  Published    1995 
  Keywords    DNA, Methylparathion, Melting Curves, Circular Dichroism, Pesticides 
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 TEI-XML for    default:Reihe_C/50/ZNC-1995-50c-0820.pdf 
 Identifier    ZNC-1995-50c-0820 
 Volume    50