| | 1 | Author
| JanuszVladimír Blasiaka, Zofia Kleinwächterb, RenataŽ. Walter3, Aludováb | Requires cookie* | | | Title
| Interaction of Organophosphorus Insecticide Methylparathion with Calf Thymus D N A and a Synthetic D N A Duplex  | | | | Abstract
| The interaction of an organophosphorus insecticide m ethylparathion (O.O-dimethyl 0-4-nitrophenyl phosphorothioate) with double-stranded DNA was characterized by UV and circular dichroism (CD) spectroscopy. Two kinds of DNA were employed: calf thymus DNA (CT DNA) and a synthetic two-stranded oligomer of sequence 5'-d(TTG GA TCCG AA TT-C A A G CTT)-3\ Melting curves and CD spectra were taken for the DNAs in the presence of the insecticide at methylparathion/DNA base pair molar ratio of 0.5. The insecticide evoked a decrease of the melting tem perature and a broadening of the transition range for CT DNA. Similar effects were observed for the synthetic oligomer but they were less pronounced than in the case of CT DNA. M ethylparathion evoked a slight shift and an increase in the ampli tude of the negative band in the CD spectra of both DNAs. O btained results indicate that methylparathion may perturb the thermal stability and conformation of DNA, which is an evidence that the insecticide has an ability to interact directly with DNA. | | | |
Reference
| Z. Naturforsch. 50c, 820—823 (1995); received August 8/Septem ber 19. 1995 | | | |
Published
| 1995 | | | |
Keywords
| DNA, Methylparathion, Melting Curves, Circular Dichroism, Pesticides | | | |
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| default:Reihe_C/50/ZNC-1995-50c-0820.pdf | | | | Identifier
| ZNC-1995-50c-0820 | | | | Volume
| 50 | |
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