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1983 (1)
1981 (1)
1Author    K.-H Knobloch, B. Hansen, J. Berlin, ForschungM B H, A. Bteilung, Pflanzliche Zellkulturen, M. Ascheroder WegRequires cookie*
 Title    Medium-Induced Formation of Indole Alkaloids and Concomitant Changes of Interrelated Enzyme Activities in Cell Suspension Cultures of Catharanthus roseus  
 Abstract    Recently m edium conditions have been developed which stim ulate the form ation of the indole alkaloid ajm alicine in cell suspension cultures o f Catharanthus roseus [6]. W hen cells were subjected to these conditions the alkaloid accum ulation was preceded by a 12-fold increase o f the specific activity o f tryptophan decarboxylase. The enzyme activity showed a maxim um two days after the cell transfer into the induction medium and subsequently declined. In contrast the activity of strictosidine synthase, the enzyme condensing tryptam ine and secologanin, was present over the entire measuring period at a constant level. The intracellular content of tryp­ tamine and ajmalicine increased during a period of 6 days after cell transfer and reached a plateau after that time. A possible regulatory function o f tryptophan decarboxylase in indole alkaloid biosynthesis is discussed. 
  Reference    Z. Naturforsch. 36c, 40—4 (1981); received N ovem ber 3 1980 
  Published    1981 
  Keywords    Catharanthus roseus, Indole Alkaloids, Tryptophan Decarboxylase, Strictosidine Synthase, Media Effects 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0040.pdf 
 Identifier    ZNC-1981-36c-0040 
 Volume    36 
2Author    Jochen Berlin, Edgar Forche, Victor Wray, Joachim Hammer, Wolfgang HöselRequires cookie*
 Title    Formation of Benzophenanthridine Alkaloids by Suspension Cultures o f Eschscholtzia californica  
 Abstract    Suspension cultures of Eschscholtzia californica accum ulate the dihydro forms o f the benzo­ phenanthridine alkaloids sanguinarine, chelirubine, macarpine and chelerythrine, all o f which are known to be constituents o f the Eschscholtzia plant. U nder most experimental conditions dihydrochelirubine was found to be the main constituent of the cultured cells. The specific yields of alkaloids varied from zero to 1.7% on a dry weight basis depending on the m edia conditions. The highest specific yield was 1.5 m g/g dry weight or 13 mg/1 with the growth m edium B5. After transfer of the cells into the induction medium IM2 the alkaloid accum ulation increased to 17 mg/g dry weight and 146 mg/1. The induction m edium contained increased levels o f sucrose, decreased levels of phosphate and was devoid of phytohormones. The effect o f the various m edia conditions on the biosynthesis of phenolics was quite different to those found for the alkaloids. 
  Reference    Z. Naturforsch. 38c, 346 (1983); received February 7 1983 
  Published    1983 
  Keywords    Eschscholtzia californica, Suspension Cultures, Benzophenanthridine Alkaloids, Phenolics, Media Effects 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0346.pdf 
 Identifier    ZNC-1983-38c-0346 
 Volume    38