| | 1 | Author
| Alfons Radunz, Renate Meier | Requires cookie* | | | Title
| Binding o f Antibodies onto the Thylakoid Membrane VII. Localization o f Coupling Factor o f Photophosphorylation in the Lamellar System o f Chloroplasts from Antirrhinum majus  | | | | Abstract
| The maximal binding o f antibodies against the subunits o f the coupling factor o f photo phosphorylation onto the ultrasonic sediment and to stroma-freed chloroplasts o f Antirrhinum majus was determined. Different surfaces o f the thylakoid membrane are accessible to antibodies in both chloroplast preparations. Stroma-freed chloroplasts bind antibodies only at the outer sur face, which is directed towards the stroma in intact chloroplasts. In the ultrasonic sediment, which is the product of ultrasonication and centrifugation, as was demonstrated by electronmicroscopy, the major part o f the surface directed towards the inside o f the thylakoids is accessible. Both chloroplasts preparations are able to bind different amounts o f antibodies to the five sub units of the coupling factor. While antigenic determinants o f all five subunits are present on the surface directed to the outside, the «^-components seems to be lacking on the surface directed | | | |
Reference
| Z. Naturforsch. 37c, 236 (1982); received December 3 1981 | | | |
Published
| 1982 | | | |
Keywords
| Chloroplasts, Thylakoid Membrane, Subunits o f the Coupling Factor, Antibodies, Maximal Binding o f Antibodies | | | |
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| default:Reihe_C/37/ZNC-1982-37c-0236.pdf | | | | Identifier
| ZNC-1982-37c-0236 | | | | Volume
| 37 | |
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