| | 1 | Author
| R. R. Br, G. Spribille, Forkm | Requires cookie* | | | Title
| Genetic Control of Chalcone Synthase Activity in Flowers of Matthiola incana  | | | | Abstract
| Chalcone synthase activity was demonstrated in enzyme preparations from flowers o f defined genotypes of Matthiola incana (stock). The product formed from 4-coumaroyl-CoA and malonyl-CoA was naringenin and not the isomeric chalcone, because chalcone isomerase was also present in the reaction mixture. Chalcone synthase activity could be detected only in flower extracts o f genotypes with wild-type alleles at the locus f Thus, the interruption of the anthocyanin pathway in white flowering lines with recessive alleles (ff) of this gene is clearly due to a lack o f this enzyme activity. Independent on the genetic state of the locus b which controls the formation of pelargonidin or cyanidin, respectively, in the flowers, 4-coumaroyl-CoA was the only suitable substrate for the condensation reaction. | | | |
Reference
| Z. Naturforsch. 36c, 619 (1981); received April 3 1981 | | | |
Published
| 1981 | | | |
Keywords
| Anthocyanins, Flavonoids, Chalcone Synthase, Genetic Control, Matthiola incana | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0619.pdf | | | | Identifier
| ZNC-1981-36c-0619 | | | | Volume
| 36 | |
| 2 | Author
| V. Hemleben | Requires cookie* | | | Title
| Anthocyanin Carrying Structures in Specific Genotypes of Matthiola incana R. Br | | | | Abstract
| Several lines of the genetically well defined Matthiola incana (Brassicaceae) concerning the anthocyanin biosynthesis (line 02, 06, 10, and 14) store the anthocyanin pigment as crystals (line 06) or in anthocyanoblasts ("balls") in the epidermal cells (line 02, 10, and 14) o f the flower petals. The genetic constitution o f these genotypes is in addition to the basic factors f +, g+, and e+, b+b+ or bb (cyanidin or pelargonidin type), 11, u+u+, and v+v+ or vv, demonstrating that the 11, u+u+ constitution is responsible for precipitation of anthocyanins in a certain structure indepen dent of the other modificationally acting genes b and v. The 11, u+u+ types accumulate preferentially acylated 3-biosides. | | | |
Reference
| Z. Naturforsch. 36c, 925 (1981); received September 29 1981 | | | |
Published
| 1981 | | | |
Keywords
| Anthocyanin, 3-Biosides, Crystals, Matthiola incana, Brassicaceae | | | |
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| default:Reihe_C/36/ZNC-1981-36c-0925.pdf | | | | Identifier
| ZNC-1981-36c-0925 | | | | Volume
| 36 | |
| 4 | Author
| G. Forkm, W. Heller, H. Grisebach | Requires cookie* | | | Title
| Anthocyanin Biosynthesis in Flowers of Matthiola incana Flavanone 3-and Flavonoid 3'-Hydroxylases | | | | Abstract
| Enzyme preparations from flowers o f defined genotypes o f Matthiola incana contain two dif ferent hydroxylases for hydroxylation of naringenin in the 3-and 3'-position, respectively. The 3-hydroxylase is a soluble enzyme and requires as cofactors 2-oxoglutarate, Fe2+ and ascorbate. Besides naringenin eriodictyol is a substrate for the 3-hydroxylase. The 3'-hydroxylase is localized in the microsomal fraction and requires NADPH as cofactor. Naringenin and dihydro-kaempferol but not 4-coumarate or 4-coumaroyl-CoA are substrates for this enzyme. 3'-Hy-droxylase activity is present only in genetic lines of M. incana with the wild-type allele b +. | | | |
Reference
| Z. Naturforsch. 35c, 691—6 (1980); received June 25 1980 | | | |
Published
| 1980 | | | |
Keywords
| Anthocyanins, Flavonoids, Biosynthesis, Flavanon 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Matthiola incana | | | |
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| default:Reihe_C/35/ZNC-1980-35c-0691.pdf | | | | Identifier
| ZNC-1980-35c-0691 | | | | Volume
| 35 | |
| 5 | Author
| B. D. Angelm Ayr, G. Stotz, R. Spribille, O. Rk | Requires cookie* | | | Title
| Relationship between Flower Development, Anthocyanin Accumulation and Activity o f Enzymes Involved in Flavonoid Biosynthesis in Matthiola incana R. Br | | | | Abstract
| The activity of five enzymes concerning anthocyanin biosynthesis as well as the anthocyanin accumulation were studied during the developm ent o f buds and flowers o f Matthiola incana. The investigations included the first three enzymes in the anthocyanin pathw ay, chalcone synthase, chalcone isomerase and flavanone 3-hydroxylase, and the flavonoid-m odifying enzymes, flavo-noid 3'-hydroxylase and flavonoid 3-O-glucosyltransferase. The bud and flower development was subdivided into eight stages with respect to m orphological criteria. On a fresh weight basis, a substantial correlation between anthocyanin content and the activities o f all o f the five enzymes were found in the various developmental stages. Furtherm ore, the anthocyanins formed are obviously not or only less subject to degradation. Although all m axim a of activity proved to be in buds, clear differences were observed between the five enzymes with regard to increase and stage of maximum activity. The isolation of other enzymes involved in flavonoid biosynthesis is likely to be most successful in the bud stages. | | | |
Reference
| Z. Naturforsch. 38c, 551—555 (1983); received April 111983 | | | |
Published
| 1983 | | | |
Keywords
| Matthiola incana, Anthocyanin Biosynthesis, Anthocyanin Content, Enzyme Activities, Flower Development | | | |
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| default:Reihe_C/38/ZNC-1983-38c-0551.pdf | | | | Identifier
| ZNC-1983-38c-0551 | | | | Volume
| 38 | |
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