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1	Author	Y. Yamazaki, M.-R Kula	Requires cookie*
	Title	Entwicklung neuer Plattentests zum Nachweis mikrobieller Hydrolysen von Estern und Oxidationen von 2-Hydroxycarbonsäuren Developm ent of New Plate Tests for the D etection of Microbial Hydrolysis of Esters and Oxidations of 2-HydroxycarboxyIic Acids
	Abstract	The application o f the thin-agar-layer coated filter culture technique (Z. Naturforsch. 42c, 1082 (1987)) has been extended to the detection of ester hydrolysis and 2-hydroxyacid oxidation by microbial colonies. The former was perform ed by spraying with brom ocresol purple and the latter with a salicylhydrazide reagent. Under the optim ized conditions, the hydrolysis activity of more than 20 [imol/h • g-wet cells and the oxidation activity o f more than 40 \|j.mol/h • g-wet cells were usually detected directly on the filter-plate cultures o f bacteria, yeasts and molds.
	Reference	Z. Naturforsch. 42c, 1187—1192 (1987); received July 30 1987
	Published	1987
	Keywords	Esterase, M icrobial, O xidation, Indicator, Salicylhydrazide
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	TEI-XML for	default:Reihe_C/42/ZNC-1987-42c-1187.pdf
	Identifier	ZNC-1987-42c-1187
	Volume	42

2	Author	Yoshim Itsu Yamazaki, AkihikoM. Aruyam, Kuniaki Hosono, Takanori Higashihara, HarumI. Kobayashi	Requires cookie*
	Title	Asymmetrie Reduction of Synthetic Ketones by Marine Microorganisms
	Abstract	Three strains o f bacteria reducing (trifluoroacetyl)ferrocene (3) to optically pure (R)-2,2,2-trifluoro-l-hydroxyethylferrocene (4) and one bacterial strain reducing 3 to (5^)-4 o f moderate optical purity were isolated from sea-water collected at the coastal areas in Ibaraki prefecture o f Japan. The former three strains were identified as Micrococcus lylae, M icrococcus luteus, and D eleya marina and the latter as Bacillus licheniformis. These strains also asymmetrically reduced som e other synthetic ketones, e.g., 2,2,2-trifluoroacetophenone (7) and phenyl tri-methylsilyl ketone (9). Further screening o f microorganisms capable o f reducing 3 was done with bacteria isolated from sea-water o f the deep sea (Okinawa trough, Japan trench, and Mariana trough) and o f the pelagic areas (Indian Ocean and South China Sea). M ost o f these marine strains preferentially reduced 3 to (/?)-4 similar to the coastal strains, but the frequency o f finding very highly enantioselective strains (i.e., those forming 4 o f > 90% e.e.) was remark ably high in several sites o f the deep sea and pelagic areas as compared with the coast and terrestrial environment.
	Reference	Z. Naturforsch. 48c, 451 (1993); received December 23 1992/February 24 1993
	Published	1993
	Keywords	M icrobial, Asymmetric, Reduction, Marine, M icroorganism
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	TEI-XML for	default:Reihe_C/48/ZNC-1993-48c-0451.pdf
	Identifier	ZNC-1993-48c-0451
	Volume	48