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1993 (1)
1987 (1)
1Author    Y. Yamazaki, M.-R KulaRequires cookie*
 Title    Entwicklung neuer Plattentests zum Nachweis mikrobieller Hydrolysen von Estern und Oxidationen von 2-Hydroxycarbonsäuren Developm ent of New Plate Tests for the D etection of Microbial Hydrolysis of Esters and Oxidations of 2-HydroxycarboxyIic Acids  
 Abstract    The application o f the thin-agar-layer coated filter culture technique (Z. Naturforsch. 42c, 1082 (1987)) has been extended to the detection of ester hydrolysis and 2-hydroxyacid oxidation by microbial colonies. The former was perform ed by spraying with brom ocresol purple and the latter with a salicylhydrazide reagent. Under the optim ized conditions, the hydrolysis activity of more than 20 [imol/h • g-wet cells and the oxidation activity o f more than 40 |j.mol/h • g-wet cells were usually detected directly on the filter-plate cultures o f bacteria, yeasts and molds. 
  Reference    Z. Naturforsch. 42c, 1187—1192 (1987); received July 30 1987 
  Published    1987 
  Keywords    Esterase, M icrobial, O xidation, Indicator, Salicylhydrazide 
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 TEI-XML for    default:Reihe_C/42/ZNC-1987-42c-1187.pdf 
 Identifier    ZNC-1987-42c-1187 
 Volume    42 
2Author    Yoshim Itsu Yamazaki, AkihikoM. Aruyam, Kuniaki Hosono, Takanori Higashihara, HarumI. KobayashiRequires cookie*
 Title    Asymmetrie Reduction of Synthetic Ketones by Marine Microorganisms  
 Abstract    Three strains o f bacteria reducing (trifluoroacetyl)ferrocene (3) to optically pure (R)-2,2,2-trifluoro-l-hydroxyethylferrocene (4) and one bacterial strain reducing 3 to (5^)-4 o f moderate optical purity were isolated from sea-water collected at the coastal areas in Ibaraki prefecture o f Japan. The former three strains were identified as Micrococcus lylae, M icrococcus luteus, and D eleya marina and the latter as Bacillus licheniformis. These strains also asymmetrically reduced som e other synthetic ketones, e.g., 2,2,2-trifluoroacetophenone (7) and phenyl tri-methylsilyl ketone (9). Further screening o f microorganisms capable o f reducing 3 was done with bacteria isolated from sea-water o f the deep sea (Okinawa trough, Japan trench, and Mariana trough) and o f the pelagic areas (Indian Ocean and South China Sea). M ost o f these marine strains preferentially reduced 3 to (/?)-4 similar to the coastal strains, but the frequency o f finding very highly enantioselective strains (i.e., those forming 4 o f > 90% e.e.) was remark­ ably high in several sites o f the deep sea and pelagic areas as compared with the coast and terrestrial environment. 
  Reference    Z. Naturforsch. 48c, 451 (1993); received December 23 1992/February 24 1993 
  Published    1993 
  Keywords    M icrobial, Asymmetric, Reduction, Marine, M icroorganism 
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 TEI-XML for    default:Reihe_C/48/ZNC-1993-48c-0451.pdf 
 Identifier    ZNC-1993-48c-0451 
 Volume    48