| | 1 | Author
| Kaoru Kubota, Xiao-Ni Li1, Hiroshi Ashihara | Requires cookie* | | | Title
| The Short-Term Effects of Inorganic Phosphate on the Levels of Metabolites in Suspension-Cultured Catharanthus roseus Cells  | | | | Abstract
| In order to analyze the effects o f inorganic phosphate (Pi) on the energy metabolism o f suspen sion-cultured cells o f Catharanthus roseus, short-term changes in levels o f glycolytic m etabolites were m onitored after the addition o f 1.25 mM Pi to cultures of cells previously cultured in a Pi-free M urashige-Skoog m edium for 24 h. The levels o f all phosphorylated interm ediates o f glycolysis exam ined were found to increase after addition o f Pi. The most striking increases were observed in levels o f glucose-6-phosphate and fructose-6-phosphate which rose to approximately 8 (after 30 min) and 15 tim es (after 120 min) their initial values. The levels o f A TP and pyrophosphate doubled during the first 30 min o f incubation. In contrast, the level of pyruvate decreased signifi cantly during the initial 30 min and then began to increase again for the next 90 min. The short term effects o f Pi on the rates o f synthesis o f proteins and R N A were estim ated from the rates o f incorporation o f 14C-labelled am ino acids and uridine into the macrom olecules. No appreciable stim ulation o f the synthesis o f proteins and R N A was induced by Pi within 120 min after the addition o f Pi. Possible m echanism s are discussed that may be involved in the changes in m etab o lism initiated by the addition o f Pi to the cultures. | | | |
Reference
| Z. Naturforsch. 44c, 802—806 (1989); received June 5 1989 | | | |
Published
| 1989 | | | |
Keywords
| Catharanthus roseus, Suspension Culture, Inorganic Phosphate, G lycolytic M etabolites, M etabolic Regulation | | | |
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| default:Reihe_C/44/ZNC-1989-44c-0802.pdf | | | | Identifier
| ZNC-1989-44c-0802 | | | | Volume
| 44 | |
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