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1984 (1)
1Author    AugustoF. Garcia, G.Erhart DrewsRequires cookie*
 Title    Properties of Membrane Fractions Prepared by Chromatophore-Liposome Fusion  
 Abstract    Intracytoplasmic membrane vesicles (chromatophores) isolated from Rhodopseudomonas capsulata cells were fused with liposom es by a pH transition procedure. Vesicles o f lower density and higher lipid contents and larger diam eter than chrom atophores were obtained. Similar results were observed by Ca2+ induced fusion and by the freeze-thawing method. Respiratory and light-induced electron transport were m easured in chrom atophores and fused vesicles. Light-induced reaction center bleaching was observed in all types o f vesicles, whereas repiratory electron transport was substantially dim inished by lipid incorporation. U biquinone 10 restored to some extent respiratory electron transport and oxidative phosphorylation and it m odified the photophosphorylation kinetics under continuous light. Electrochrom ic carotenoid band-shift and the 9-aminoacridine fluorescence quenching indicate that the capacity o f the fused vesicles to maintain an electrochemical proton gradient has not been substantially dim inished. From the kinetics o f 9-aminoacridine quenching an increased K+-perm eability seem s to be apparent. 
  Reference    Z. Naturforsch. 39c, 1112—1119 (1984); received July 23 1984 
  Published    1984 
  Keywords    Photosynthetic Apparatus, Respiratory Chain, Rhodopseudom onas capsulata, M embrane Fusion, Liposomes, Photophosphorylation 
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 TEI-XML for    default:Reihe_C/39/ZNC-1984-39c-1112.pdf 
 Identifier    ZNC-1984-39c-1112 
 Volume    39