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'Lysosomes' in keywords Facet   section ZfN Section C  [X]
Facet   Publication Year 1979  [X]
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1979[X]
1Author    Florenz Sasse, Dietlinde Backs-Hüsemann, Wolfgang BarzRequires cookie*
 Title    Isolation and Characterization of Vacuoles from Cell Suspension Cultures of Daucus carota  
 Abstract    A reliable procedure for the isolation o f vacuoles from anthocyanin-containing cells o f Daucus carota cell suspension cultures has been developed. From cells o f the late linear growth phase, protoplasts were prepared and purified in a sucrose/sorbitol gradient. Vacuoles were liberated from these protoplasts by osm otic shock and purified in a M etrizamide step gradient. The vacuole-containing fractions were analysed for their anthocyanin content as a measure for the yield o f vac­ uoles. The purity o f vacuoles was examined by assaying various marker enzym es in both proto­ plasts and vacuoles. The purest vacuolar fraction had 8% o f the total activity o f glucose-6-phos-phate dehydrogenase (marker for cytosol), 8% o f cytochrom e oxidase (m itochondria) and 10% o f N A DPH -cytochrom e c reductase (ER). 55% o f the acid phosphatase activity o f the protoplasts and 35% o f the total malate were recovered in the vacuoles. The vacuolar pool o f am ino acids is quite large. D ata for 15 am ino acids show that 44 to 73% are being located in the vacuole. 
  Reference    Z. Naturforsch. 34c, 848—853 (1979); received M ay 15 1979 
  Published    1979 
  Keywords    Daucus carota, Protoplasts, Vacuoles, Lysosomes, A m ino Acids, Anthocyanin 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0848.pdf 
 Identifier    ZNC-1979-34c-0848 
 Volume    34