| | 2 | Author
| Florenz Sasse, Dietlinde Backs-Hüsemann, Wolfgang Barz | Requires cookie* | | | Title
| Isolation and Characterization of Vacuoles from Cell Suspension Cultures of Daucus carota  | | | | Abstract
| A reliable procedure for the isolation o f vacuoles from anthocyanin-containing cells o f Daucus carota cell suspension cultures has been developed. From cells o f the late linear growth phase, protoplasts were prepared and purified in a sucrose/sorbitol gradient. Vacuoles were liberated from these protoplasts by osm otic shock and purified in a M etrizamide step gradient. The vacuole-containing fractions were analysed for their anthocyanin content as a measure for the yield o f vac uoles. The purity o f vacuoles was examined by assaying various marker enzym es in both proto plasts and vacuoles. The purest vacuolar fraction had 8% o f the total activity o f glucose-6-phos-phate dehydrogenase (marker for cytosol), 8% o f cytochrom e oxidase (m itochondria) and 10% o f N A DPH -cytochrom e c reductase (ER). 55% o f the acid phosphatase activity o f the protoplasts and 35% o f the total malate were recovered in the vacuoles. The vacuolar pool o f am ino acids is quite large. D ata for 15 am ino acids show that 44 to 73% are being located in the vacuole. | | | |
Reference
| Z. Naturforsch. 34c, 848—853 (1979); received M ay 15 1979 | | | |
Published
| 1979 | | | |
Keywords
| Daucus carota, Protoplasts, Vacuoles, Lysosomes, A m ino Acids, Anthocyanin | | | |
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| default:Reihe_C/34/ZNC-1979-34c-0848.pdf | | | | Identifier
| ZNC-1979-34c-0848 | | | | Volume
| 34 | |
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