| | 1 | Author
| Katarzyna Woźniak, Zofia Walter | Requires cookie* | | | Title
| Induction of DNA-Protein Cross-Links by Platinum Compounds  | | | | Abstract
| The differences between cis-and mws-diamminedichloroplatinum II (DDP) in forming DNA-protein cross-links in isolated human lymphocytes were investigated. Both cis-and rra/75-DDP can induce DNA-protein cross-links. We show that cis-DDP forms complexes between D N A and proteins faster than trans-DDP. This results from an increase in the quan tity of D NA and platinum together with an increase in drug concentration. Under the same conditions trans-DDP causes a decrease in DNA-forming complexes with proteins. After a 12 h incubation of lymphocytes we observe a similar level of DNA in DNA-protein cross links induced by D D P isomers, but more platinum appears in complexes induced by trans-DDP. The results obtained demonstrate that the antitumor drug -ds-DDP and the clinically ineffective trans-DDP induce links between D N A and proteins in a different manner. We suggest that the therapeutic activity of ds-DDP can in part arise from rapidly forming DNA-protein complexes which can destroy the most important cellular processes, such as replica tion and transcription. | | | |
Reference
| Z. Naturforsch. 55c, 731—736 (2000); received March 30/June 26 2000 | | | |
Published
| 2000 | | | |
Keywords
| Lymphocytes, DNA-Protein, Cross-Links | | | |
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| default:Reihe_C/55/ZNC-2000-55c-0731.pdf | | | | Identifier
| ZNC-2000-55c-0731 | | | | Volume
| 55 | |
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