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1Author    F. Ojeda, M. Flores, H. FolchRequires cookie*
 Abstract    The effect o f irradiation on the anti Ig binding ability o f lymphocytes after irradiation was studied. Norm al control and irradiated lym phoid cells were treated with rabbit anti mouse IgG fluorescein conjugated serum and the fluores­ cein positive cells compared. A reduction in the proportion o f stained cells in the irradiated lym phocytes was found. It depends on the dose and on the incubation tim e at 37 °C after irradiation. The m axim um effect was detected after 10 minutes o f incubation, longer incubation is associated with a partial recovery o f the stainability o f the IgG recep­ tors. 
  Reference    Z. Naturforsch. 34c, 888 (1979); received January 30 1979 
  Published    1979 
  Keywords    Lymphocytes, Receptors, Irradiation 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0888_n.pdf 
 Identifier    ZNC-1979-34c-0888_n 
 Volume    34 
2Author    Katarzyna Woźniak, Zofia WalterRequires cookie*
 Title    Induction of DNA-Protein Cross-Links by Platinum Compounds  
 Abstract    The differences between cis-and mws-diamminedichloroplatinum II (DDP) in forming DNA-protein cross-links in isolated human lymphocytes were investigated. Both cis-and rra/75-DDP can induce DNA-protein cross-links. We show that cis-DDP forms complexes between D N A and proteins faster than trans-DDP. This results from an increase in the quan­ tity of D NA and platinum together with an increase in drug concentration. Under the same conditions trans-DDP causes a decrease in DNA-forming complexes with proteins. After a 12 h incubation of lymphocytes we observe a similar level of DNA in DNA-protein cross­ links induced by D D P isomers, but more platinum appears in complexes induced by trans-DDP. The results obtained demonstrate that the antitumor drug -ds-DDP and the clinically ineffective trans-DDP induce links between D N A and proteins in a different manner. We suggest that the therapeutic activity of ds-DDP can in part arise from rapidly forming DNA-protein complexes which can destroy the most important cellular processes, such as replica­ tion and transcription. 
  Reference    Z. Naturforsch. 55c, 731—736 (2000); received March 30/June 26 2000 
  Published    2000 
  Keywords    Lymphocytes, DNA-Protein, Cross-Links 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0731.pdf 
 Identifier    ZNC-2000-55c-0731 
 Volume    55 
3Author    Mircea Popescu, Jürgen Löhler, Fritz Lehmann-GrubeRequires cookie*
 Abstract    During persistent infection of mice with the lymphocytic choriomeningitis (LCM) virus approximately 3% of leuko­ cytes were found to contain viral antigen and to produce infectious virus. Morphologically, infected cells were shown not to be lymphoblasts and their numbers were not reduced by removal of mononuclear phagocytes. We conclude that in LCM virus carrier mice true lymphocytes participate in the infectious process. 
  Reference    (Z. Naturforsch. 32c, 1026 [1977]; received October 7 1977) 
  Published    1977 
  Keywords    Lymphocytic Choriomeningitis Virus Carrier Mice, Persistent Infection, Lymphocytes 
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 TEI-XML for    default:Reihe_C/32/ZNC-1977-32c-1026_n.pdf 
 Identifier    ZNC-1977-32c-1026_n 
 Volume    32 
4Author    H. Rainer, M. Aurer, Reinhard MaschlerRequires cookie*
 Title    The Influence of Spermine, Spermidine and Various Sera on T-Lymphocyte and Granulocyte Colony Growth in vitro  
 Abstract    Using T-lymphocyte (T-LC) and granulocyte colony (GC) assays with truly proliferating cells, the dose-response relationships of spermine and spermidine, of fetal calf (FCS), calf (CS), horse (HS) and human AB serum (A B S), and of the polyamines in the presence of selected does of the sera were analyzed. In contrast to earlier observations using [3H] thymidine uptake measure­ ments it was found that the polyamines as well as the sera themselves inhibit T-LC and GC growth in the presence of autologous HS and ABS, respectively. The polyamines also inhibit in the presence of additional HS and ABS, yet most effectively with added FCS and CS. Thus the previously reported species-specificity of the serum-dependent polyamine inhibition has here been shown to be more quantitative than qualitative. These studies stress the significance of assays utilizing truly dividing cells and broad dose-response relationships in order to assess specific biological effects in vitro. 
  Reference    Z. Naturforsch. 34c, 452 (1979); received January 16 1979 
  Published    1979 
  Keywords    Spermine, Spermidine, Human Serum, Lymphocyte, Granulocyte Colony Growth 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0452.pdf 
 Identifier    ZNC-1979-34c-0452 
 Volume    34 
5Author    S. Mazzuca, L. SportelliRequires cookie*
 Title    Thermal Behaviour of Lymphocyte Membrane: ESR Investigation  
 Abstract    The order parameter, S, of the plasma membrane of in toto human peripheral blood lym­ phocytes was obtained by electron spin resonance spectroscopy in the temperature range 25-41 C. This membrane is completely in the liquid crystalline state above 31 C. In presence of the antigen ETB from Staphylococcus aureus at the concentration of 4 (ig/3 * 107 cells an overall decrease of the order parameter for this membrane is observed. The decrease of 5 is followed by an upwards shift at about 35 C of the temperature of the liquid crystalline state. 
  Reference    Z. Naturforsch. 45c, 1060—1062 (1990); received April 6 1990 
  Published    1990 
  Keywords    Plasma Membrane, Lymphocyte, Phase Transition, Staphylococcal Enterotoxin B, Spin Labeling 
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 TEI-XML for    default:Reihe_C/45/ZNC-1990-45c-1060.pdf 
 Identifier    ZNC-1990-45c-1060 
 Volume    45 
6Author    M. Anfred Reitza, PeterG. UtjahrbRequires cookie*
 Title    Acid DNase Activities in Peripheral, Mononuclear Blood Cells: A Possible Parameter to Detect Proliferating Cell Populations  
 Abstract    After electrophoresis in D N A -containing polyacrylamide gels, two acid D N ase activities can be detected in peripheral, mononuclear cells o f the human blood. One o f these acid D N ase activities correlates with cell proliferation; its isoelectrical point is at pi 7.4. By means o f this D N ase activity, a quantity o f less than 1% leukemic cells can be detected. The increased acid D N ase activity can indicate the proliferation o f malignant cell populations and possibly the proliferation o f cell populations during im m unological reactions. 
  Reference    Z. Naturforsch. 47c, 910 (1992); received September 7 1992 
  Published    1992 
  Keywords    Lymphocytes, Leukemic Cells, Acid D N ase Activities, Proliferating Cell Populations 
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 TEI-XML for    default:Reihe_C/47/ZNC-1992-47c-0910.pdf 
 Identifier    ZNC-1992-47c-0910 
 Volume    47