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1983 (1)
1981 (1)
1Author    R. Maschler, C. J. Smith, J. C. Allen, H. R. MaurerRequires cookie*
 Title    Elucidation of the Different Effects of Polyamines and Other Naturally-Occurring Inhibitors of Cell Proliferation (Chalones) on T-Lymphocyte and Granulocyte Colony Growth in vitro  
 Abstract    Using T-lymphocyte and granulocyte colony assays with truly proliferating cells the effects of the polyamine spermine and o f other naturally-occurring inhibitors of cell proliferation have been differentiated. It has been confirmed that spermine, in the presence o f fetal calf serum, is a potent inhibitor of cell proliferation. This inhibition could be reversed by the addition o f either 3-hydroxybenzyl-oxyamine or 4-bromo-3-hydroxybenzyl-oxyamine, both of which are inhibitors of the polyamine oxidase. In comparison, fractions isolated from calf thymus were shown to inhibit lymphocyte, but not granulocyte colony growth, indicating their tissue specificity and lymphocyte chalone activity. Further this inhibition was not reversed by polyamine oxidase inhibitors demonstrating that polyamines were not the inhibitory principles in this preparation. 
  Reference    Z. Naturforsch. 38c, 74—7 (1983); received February 24/August 24 1982 
  Published    1983 
  Keywords    Polyamines, Lymphocyte Responsiveness, Polyamine Oxidase Inhibitors, Lymphocyte Chalone 
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 TEI-XML for    default:Reihe_C/38/ZNC-1983-38c-0074.pdf 
 Identifier    ZNC-1983-38c-0074 
 Volume    38 
2Author    Reinhard Maschler, H.Rainer MaurerRequires cookie*
 Title    Lymphocyte Chalone from Calf Thymus: Problems of Large Scale Extraction and Assay  
 Abstract    Large scale extraction and assay of the lymphocyte chalone (LC), the natural lymphocyte proliferation inhibitor, require optimized, reproducible and standardizable methods. To this end we compared the effects on LC yield of storing thymus at different temperatures after collection from calves: Both quick freezing on solid C 0 2 and slow freezing at -5 0 °C led to a 30% loss o f LC yield relative to that from unfrozen tissue kept at 0°C . Moreover we found that the apparent decrease or total loss of LC activity upon storage of a purified LC fraction may result from an occasional lack of LC-responsiveness of human blood lymphocytes depending on the donor's physiological state, in spite of normal PHA reactivity. This suggested the use o f LC-responsive, cryopreserved lymphocytes, the advantages of which are documented and discussed in detail. 
  Reference    Z. Naturforsch. 36c, 568—571 (1981); received March 4/April 24 1981 
  Published    1981 
  Keywords    Lymphocyte Chalone, Calf Thymus Freezing, Lymphocyte Responsiveness, Cryopreservation, Colony Assay 
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 TEI-XML for    default:Reihe_C/36/ZNC-1981-36c-0568.pdf 
 Identifier    ZNC-1981-36c-0568 
 Volume    36