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'Lupinus polyphyllus' in keywords Facet   section ZfN Section C  [X]
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1991 (1)
1980 (1)
1979 (1)
1Author    M. Wink, T. Hartmann, L. WitteRequires cookie*
 Title    Enzymatic Synthesis of Quinolizidine Alkaloids in Lupin Chloroplasts  
 Abstract    The enzymatic sequence responsible for the biosynthesis o f tetracyclic quinolizidine alkaloids could be localized in chloroplasts isolated from Lupinus polyphyllus leaves and L. albus seedlings by differential centrifugation. U pon feeding of cadaverine to isolated chloroplasts lupanine is produced as the m ain alkaloid. Chloroplasts treated with digitonine produce sparteine and 17-oxosparteine instead o f lupanine, thus indicating that the biosynthetic sequence is interrupted. The intermediacy of 17-oxosparteine could be confirmed since exogeneous 17-oxosparteine is con­ verted into lupanine by intact chloroplasts. 17-Oxosparteine synthase (see Z. N aturforsch. 34 c, 704 1979) the key enzyme of quinolizidine alkaloid biosynthesis could be solubilized from chloro­ plasts treated with detergents or osmotic shock. 
  Reference    Z. Naturforsch. 35c, 93—9 (1980); received Septem ber 26 1979 
  Published    1980 
  Keywords    Quinolizidine Alkaloids, Enzymatic Synthesis, Isolated Chloroplasts, Lupinus polyphyllus 
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 TEI-XML for    default:Reihe_C/35/ZNC-1980-35c-0093.pdf 
 Identifier    ZNC-1980-35c-0093 
 Volume    35 
2Author    H. Ans-M, Artin SchiebelRequires cookie*
 Title    A Model Mechanism for the Enzymatic Synthesis of Lupin Alkaloids  
 Abstract    A crude enzyme preparation obtained from cell suspension cultures o f Lupinus polyphyllus cat­ alyzes the pyruvate dependent conversion o f cadaverine into the tetracyclic lupin alkaloids. As the first reaction product 17-oxosparteine could be identified by gas-liquid chrom atography and mass spectroscopy. In som e experiments sparteine was found additionally. A participation o f di­ amine oxidase could be ruled out. The cadaverine-pyruvate transaminating enzyme system (17-oxosparteine synthase) catalyzes the formation o f 17-oxosparteine from three cadaverine units without releasing free intermediates. These results are inconsistent with the hypothetical m echa­ nism thus far formulated for the lupin alkaloid biosynthesis. A new enzym atic model mechanism is proposed regarding both the results o f the enzymatic experiments and those o f the in vivo tracer studies. 
  Reference    Z. Naturforsch. 34c, 704—708 (1979); received June 8 1979 
  Published    1979 
  Keywords    Lupinus polyphyllus, Cell Suspension Cultures, Q uinolizidine Alkaloids, Enzymatic Synthesis, Biosynthetic Model Mechanism 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-0704.pdf 
 Identifier    ZNC-1979-34c-0704 
 Volume    34 
3Author    J. Berlinab, L. Fecker, C. R. Ügenhagenab, C. S. Atorc, D. S. Trackd, L. W. Itted, V. W. RaybRequires cookie*
 Title    Isoflavone Glycoside Formation in Transformed and Non-Transformed Suspension and Hairy Root Cultures of Lupinus polyphyllus and Lupinus hartwegii  
 Abstract    sides Transformed cell suspension and hairy root cultures were established by infecting seedlings o f Lupinus polyphyllus and L. hartwegii with various wild type strains o f Agrobacterium tume-faciens and A. rhizogenes. Transformation o f the cultures was confirmed either by their phyto­ hormone autotrophy, detection o f opines or southern analysis. Glueosides o f genistein and 2'-hydroxygenistein, were found to be the main secondary metabolites in normal and trans­ formed suspension cultures as well as in hairy root cultures. Although some o f the isoflavone glycosides o f the cultures were apparently new constituents o f Lupinus, they were afterwards also found in young seedlings. 
  Reference    Z. Naturforsch. 46c, 725 (1991); received April 23 1991 
  Published    1991 
  Keywords    Lupinus polyphyllus, Lupinus hartwegii, Cell Cultures, Hairy R oot Cultures, Isoflavone Gluco- 
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 TEI-XML for    default:Reihe_C/46/ZNC-1991-46c-0725.pdf 
 Identifier    ZNC-1991-46c-0725 
 Volume    46