| | 1 | Author
| Wilhelm Hasselbach, Vera Koenig | Requires cookie* | | | Title
| Low Affinity Calcium Binding Sites of the Calcium Transport ATPase of Sarcoplasmic Reticulum Membranes  | | | | Abstract
| Calcium binding sites having low affinity constants of < 103 M-1 were titrated in native sarcoplasmic reticulum vesicles as well as in lipid deprived m em branes and in the isolated calcium transport ATPase. Short time calcium binding m easurem ents and the determ ination o f the calcium binding heat allow to distinguish low affinity calcium binding sites located on the external surface of the sarcoplasmic reticulum m em branes from those present in the section o f the transport molecule directed to the vesicular space. The same num ber o f internal binding sites was found for preparations deprived of their lipid content as well as of preparations depleted o f their lipids and of their accessorial proteins. Magnesium interferes with calcium binding to the external as well as to the internal low affinity calcium binding sites. The im plications o f the existence o f the low affinity calcium binding sites in the internal section o f the calcium transport ATPase are discussed. | | | |
Reference
| Z. Naturforsch. 35c, 1012 (1980); received A ugust 27 1980 | | | |
Published
| 1980 | | | |
Keywords
| Sarcoplasmic Reticulum, Calcium Binding, Low Affinity Binding Sites | | | |
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| default:Reihe_C/35/ZNC-1980-35c-1012.pdf | | | | Identifier
| ZNC-1980-35c-1012 | | | | Volume
| 35 | |
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