| | 1 | Author
| Wilhelm Menke, Alfons Radunz, GeorgH. Schmid, Friederike Koenig, Rolf-Dieter Hirtz | Requires cookie* | | | Title
| Intermolecular Interactions of Polypeptides and Lipids in the Thylakoid Membrane  | | | | Abstract
| Intermolecular interactions between chloroplast lipids and a polypeptide fraction from thylakoids were investigated by far ultraviolet circular dichroism. The polypeptide fraction was isolated from dodecyl sulfate-containing buffers. It exhibits an average molecular weight of 24 000. The circular dichroism of this polypeptide fraction measured as mean residue ellipticity is greater in the presence of sodium dodecyl sulfate than in the absence of this detergent. This effect is reversible. Addition of sulfoquinovosyl diglyceride to the dodecyl sulfate-free solution of the polypeptide also causes an increase of the circular dichroism. This increase was only observed in the pH-range between 6.9 and 7.4. The effect of dodecyl sulfate or sulfolipid on the circular dichroism is inter preted to indicate an increase of a-helix content. Monogalactosyl diglyceride, digalactosyl di glyceride and phosphatidyl glycerol gave no reaction. The attempt to obtain a conformational analysis of the polypeptide in the different states did not yield an entirely satisfactory result. Anti sera to sulfolipid inhibit photosynthetic electron transport of stroma-freed chloroplasts in the region of light reaction I. This inhibition is restricted to the same pH-range as the non-covalent binding of sulfolipid to the polypeptides. It appears that in the cell membrane-bound metabolic processes are regulated by this pH-dependence of the sulfolipid-polypetide interactions. | | | |
Reference
| (Z. Naturforsch. 31c, 436 [1976]; received April 30 1976) | | | |
Published
| 1976 | | | |
Keywords
| Interactions, Polypeptides, Lipids, Thylakoid Membrane, Photosynthesis | | | |
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| default:Reihe_C/31/ZNC-1976-31c-0436.pdf | | | | Identifier
| ZNC-1976-31c-0436 | | | | Volume
| 31 | |
| 2 | Author
| Edward Göbel, Ruth Riessner, Peter Pohl | Requires cookie* | | | Title
| Einfluß von DCMU auf die Bildung von Lipiden und Fettsäuren und auf die Ultrastruktur von Euglena gracilis Influence of DCMU on the Formation of Lipids and Fatty Acids, and on the Ultrastructure of Euglena gracilis | | | | Abstract
| Euglena g racilis Klebs (strain Z) was grown heterotrophically in the dark for 6 days. Sub sequently, the cells were grown photoautotrophically under white fluorescent light with varying amounts of 3-(3,4-dichlorophenyl)-l,ldimethyl urea (DCMU) (1 .7 X 1 0 -9 — 1 .0 X 1 0 -5 mol/1) added | | | |
Reference
| (Z. Naturforsch. 31c, 687 [1976]; eingegangen am 9. März/20. September 1976) | | | |
Published
| 1976 | | | |
Keywords
| Euglena gracilis, Fatty Acids, Lipids, Ultrastructure, Chloroplasts, Mitochondria | | | |
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| default:Reihe_C/31/ZNC-1976-31c-0687.pdf | | | | Identifier
| ZNC-1976-31c-0687 | | | | Volume
| 31 | |
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