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'Lipids' in keywords Facet   Publication Year 1979  [X]
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1979[X]
1Author    Alfons RadunzRequires cookie*
 Title    Binding of Antibodies onto the Thylakoid Membrane. V. Distribution of Proteins and Lipids in the Thylakoid Membrane  
 Abstract    Binding of antibodies to proteins and lipids onto fragments of the thylakoid membrane was stu­ died and compared with the binding o f antibodies by stroma-freed chloroplasts. The mem brane fragments were prepared from strom a-free chloroplasts by ultrasonication and fractional cen­ trifugation. The fragments have an average diam eter of 200 A. T heir thickness corresponds to that of the thylakoid membrane. The m em brane fragments adsorb out o f an antiserum to lipids appro­ ximately the same am ount o f antibodies as out of an antiserum to proteins. In comparison to this, stroma-free chloroplasts bind 4 times more antibodies to proteins than to lipids. From this it fol­ lows that the major part o f the lipids is located in the m em brane surface which is directed towards the inside or is located inside the mem brane. As the chemical analysis has shown these results are not caused by an altered chemical composition of the m em brane fragments. Despite the fact that m em brane proteins bind considerably less protein antibodies than stroma-free chloroplasts, the antibody binding in m em brane fragment might be considerably increased for certain proteins such as a polypeptide with an apparent molecular weight 24000 and cytochro­ me f. Antibodies to the m ajor components o f the lipid mixture, such as to monogalactosyl diglyce-ride, trigalactosyl diglyceride, sulfoquinovosyl diglyceride and phosphatidyl glycerol are 3 to 4 times more bound by m em brane fragments than by stroma-free chloroplasts. From these results it is concluded that the thylakoid m em brane surface directed towards the inside is preponderently composed of lipids whereas the surface directed towards the outside consists only by 10 to 15% of lipids. 
  Reference    Z. Naturforsch. 34c, 1199—1204 (1979); received July 6 1979 
  Published    1979 
  Keywords    Chloroplasts, Thylakoid M em brane Surface, Proteins, Lipids, Antibodies 
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 TEI-XML for    default:Reihe_C/34/ZNC-1979-34c-1199.pdf 
 Identifier    ZNC-1979-34c-1199 
 Volume    34