| | 2 | Author
| M. Onika, Meyer, Christian Wilhelm | Requires cookie* | | | Title
| Reconstitution of Light-Harvesting Complexes from Chlorella fusca (Chlorophyceae) and Mantoniella squamata (Prasinophyceae)  | | | | Abstract
| D edicated to Professor W. Rüdiger (München) on the occasion o f his 60 th anniversary Reconstitution experiments o f light-harvesting complexes were performed with the green alga Chlorella fusca and the chlorophyll c-eontaining prasinophyte M antoniella squamata using a modified method according to Plumley and Schmidt [Proc. N atl. Acad. Sei. U .S.A . 84, 146 -1 5 0 (1987)]. Changing the pigment supply quantitatively or qualitatively in the reconsti tution mixture hom ologous and heterologous reconstitutes were obtained. In contrast to higher plants, light-harvesting polypeptides from green algae are able to bind the chlorophylls as well as the xanthophylls in different stoichiometries. H eterologous reconstitutes o f M . squamata polypeptides give further evidence for a rather high flexibility o f pigment recog nition and binding. This is the first report o f successful reconstitution o f a chlorophyll c-binding protein. Contrary to chlorophyll c-less light-harvesting com plexes, the reconstitu tion o f M . squamata is strongly pH-controlled. In summary, the results give evidence for a high specificity o f porphyrin ring recognition and variability in xanthophyll binding capacity. Therefore, it is suggested that at least in algal light-harvesting proteins chlorophyll organiza tion may be determined by other mechanisms than xanthophyll binding. | | | |
Reference
| Z. Naturforsch. 48c, 461 (1993); received December 12 1992/April 1 1993 | | | |
Published
| 1993 | | | |
Keywords
| Algae, Reconstitution, Light-Harvesting Complex, Photosynthesis, Pigment-Protein Inter action | | | |
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| default:Reihe_C/48/ZNC-1993-48c-0461.pdf | | | | Identifier
| ZNC-1993-48c-0461 | | | | Volume
| 48 | |
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