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2000 (1)
1Author    Ferng-Ruey Chen, Ping-Chung Liu, Kuo-Kau LeeRequires cookie*
 Title    Lethal Attribute of Serine Protease Secreted by Vibrio alginolyticus Strains in Kuruma Prawn Penaeus japonicus  
 Abstract    Toxicity of the extracellular products (E C P) and the lethal attribute of serine protease secreted by five pathogenic Vibrio alginolyticus strains from various sources in kuruma prawn Penaeus japonicus were studied. The E C P s of organisms originally isolated from diseased kuruma prawn or small abalone Haliotis diversicolor supertexta were more lethal (L D 50 value of 0.48 or 0.41 ^ig protein/g prawn) than those from diseased tiger prawn P. m onodon, yellow-fin porgy Acanthopagrus latus or horse m ackerel (L D 50 value of 0 .9 8 -1 .1 7 ^ig protein/g prawn). All the EC Ps manifested strong, weak and no activities against gelatin, sheep eryth­ rocytes and chitin, respectively. In immunodiffusion tests using rabbit antiserum to a purified 33 kDa serine protease of strain Swy against E C P of each tested strain produced one single precipitation band in each treatm ent. Furtherm ore, the serine protease was suggested to be the dominant protease secreted by V. alginolyticus strains tested since the majority of enzy­ matic activity of the respective E C P was inhibited by phenylmethanesulfonyl fluoride (PM SF). A higher inhibition of serine protease activity by PM SF resulted in lower mortality rate of the EC Ps injected into the prawns suggesting that the protease is one of the major lethal factor(s) secreted by V alginolyticus. 
  Reference    Z. Naturforsch. 55c, 94—9 (2000); received Septem ber 9/Novem ber 8 1999 
  Published    2000 
  Keywords    Extracellular Products, Lethality, Penaeus japonicus, Serine Protease, Vibrio alginolyticus 
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 TEI-XML for    default:Reihe_C/55/ZNC-2000-55c-0094.pdf 
 Identifier    ZNC-2000-55c-0094 
 Volume    55